exam 3 Flashcards
MS
mass spectrometry
GC
gas chromatography
HPLC
high performance liquid chromatography
TIC
total ion current
SIC
select ion current
WCOT
wall-coated open tubular
HETP
height equal to theoretical plate
FID
flame ionization detector
TCD
thermal conductivity detector
ECD
electron capture detector
ion exchange
analytes are electrostatically attached to permanently charged groups bonded to a resin head
analytes are electrostatically attached to permanently charged groups bonded to a resin head
ion exchange
adsorption
analytes stick to the surface of a solid stationary phase
analytes stick to the surface of a solid stationary phase
adsorption
partition
analytes dissolve in a liquid stationary phase or in a liquid bonded to or coated on a solid stationary phase
analytes dissolve in a liquid stationary phase or in a liquid bonded to or coated on a solid stationary phase
partition
gel exclusion
analytes are separated by size/shape
analytes are separated by size/shape
gel exclusion
affinity
analytes adhere to groups attached to the stationary phase via very specific molecular mechanisms
analytes adhere to groups attached to the stationary phase via very specific molecular mechanisms
affinity
Van Deemter equation: A
A = multiple path lengths (unaffected by U)
Van Deemter equation: B
B = longitudinal diffusion, constant rate (minimizes with increase in flow)
Van Deemter equation: C
C = finite mass transfer rate, equilibration time (minimizes with decrease in flow)
Who discovered chromatography?
Tswett
In order to analyze a sample by GC, what properties must the analytes possess?
boiling point less than 350 C
thermally stable up to 350 C
HPLC columns have lengths in which range?
5 to 30 cm
Open tubular GC columns typically have lengths in which range?
30 to 100 m
Which of the following detectors is most commonly used for HPLC?
UV