Exam 3 Flashcards
In which stage of the cell cycle are sister chromatids separated and pulled to the opposite poles of the cell?
Anaphase
What relieves mechanical strain that builds up (twisting forces) during replication in E. coli?
topoisomerase
Strand initiation in DNA replication is accomplished when an enzyme lays down a primer. Of what molecule is that primer composed?
RNA
A group of cells is assayed for DNA content immediately following mitosis and are found to have an average of 8 picograms of DNA per nucleus. Those cells would have _____ picograms of DNA at the end of the S phase and _____ picograms of DNA at the end of G2.
16, 16
Why is DNA replication called semiconservative?
Each daughter duplex contains one strand from the parent structure.
In human and many other eukaryotic species’ cells, the nuclear membrane has to disappear to permit
the attachment of microtubules to kinetochores
The specific site on the bacterial chromosome at which replication begins is called the ________
origin
Replication moves outward from the origin in ________ direction(s) and is said to be ________
both, bidirectional
What proteins bind selectively to single-stranded DNA and are responsible for keeping it extended and preventing it from being rewound and becoming double stranded?
single-stranded DNA binding (SSB) proteins
What type of hypothesized replication (Meselson-Stahl model) results in one duplex containing both parental strands and the other duplex containing two new strands of DNA after the two parental strands had served as templates for the two daughter strands?
conservative replication
In which direction are nucleotides laid down in the new strand while DNA is being synthesized?
5’—>3‘
The RNA primers that initiate replication are subsequently _____ and the resulting gap in the strand is _______ DNA and then sealed by _______
removed, filled in with, DNA ligase
In which direction do the DNA strands grow during replication?
one toward the replication fork and one away from the replication fork
New strand initiation during replication is carried out by an enzyme that makes a short RNA molecule that is used as a primer; the enzyme is a distinct type of RNA polymerase, called _______
Primase
Cells that are in a non-dividing state are in which phase?
G0
What are two copies of the same DNA (have the same exact sequence)?
Sister Chromatids
What are two copies of the same genes, but not always the same sequence (one from mom, one from dad)?
Homologous Chromosomes
In which stage of the cell cycle are sister chromatids separated and pulled to the opposite poles of the cell?
Anaphase
Meiosis is the separation of:
Both Sister Chromatids and Homologous Chromosomes
During replication, DNA is constructed in small segments called ________ that are rapidly linked to longer pieces of DNA synthesized earlier.
Okazaki fragments
A group of cells is assayed for DNA content immediately following mitosis and are found to have an average of 8 picograms of DNA per nucleus. Those cells would have _____ picograms of DNA at the end of the S phase and _____ picograms of DNA at the end of G2.
16, 16
The enzyme that joins the small fragments of the lagging strand together into a continuous strand is called
DNA ligase
Proteins that are involved in the regulation of the cell cycle, and that show fluctuations in concentration during the cell cycle, are called:
Cyclins
If a liver cell of an animal has 24 chromosomes total, the sperm cells from this animal will have how many chromosomes?
12