exam 3: lecture 10 photosynthesis Flashcards

1
Q

definition of photosynthesis

A

the process by which organisms use light energy to make sugar and oxygen gas from carbon dioxide and water

-occurs in plants. algae, and some prokaryotes
-anabolic (small molecules combined)
-endergonic (stores energy) “CARBON FIXATION” (a gas is fixed to a molecule/sugar
-stored as carbohydrate in their bodies

6 CO2 + 6H2O—-light energy—-> C6H12O6 + 6O2

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2
Q

structures of the chloroplasts

A

stoma: small openings for CO2 ^ O2 to pass thru
(high CO2 outside;low O2 inside)

intermediate membrane: the space between the outer and inner membrane
thylakoid: 1 flattened sac/disc
granum: many flattened sacs on top of each other
grama: all stacks

plants in temperate zones: keep stomata open-allow CO2 in during the day
plants in desert: close stomata during the day

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3
Q

tracking oxygen atoms in photosynthesis

A

the oxygen from CO2 goes to glucose and water
the oxygen from H2O goes to O2

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4
Q

redox reactions in photosynthesis

A

CO2 is reduced into C6H12O6
H2O is oxidized into O2

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5
Q

At which wavelengths do most energy absorb? what gives the leaf their green color?

A

red and blue wavelengths (not much in the middle -green/yellow)

Mg in the middle of their structure
CHO in chlorophyll b
CH3 in chlorophyll a (methyl group)

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6
Q

how does the transfer of energy to the reaction center of a photosystem work?

A

a photon’s energy is absorbed by a chlorhyl molecule where it excites the e- into high energy

-light energy absorbed by antenna pigment is transferred from one to the next until it reaches the main reaction center chlorophyll
-the energy is then captured by an electron acceptor

*no chemical reaction, energy is being transferred through physical only
resonance transfer: wavelength energy

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7
Q

what happens in photosystem II

A

light absorption:
* after reaction center, energy is transfer to P680, bringing an e- to a high energy level
*energy is then passed to an acceptor molecule and replaced with an electron from H2O=> water is broken down to release O2

atp synthesis:
*high energy e- travels down the ETC, losing energy as it does
*the released energy is used to pump H+ from the stroma into the thylakoid interior-> a gradient
*H+ pass thru ATP synthase-> ATP production (chemiosmosis)

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8
Q

what happens in photosystem I?

A

light absorption:
*e- arrive at photosyste I and join P700 in the reaction center.
*energy energy is absorbed–> e- is bring to a high energy level and transferred to an acceptor molecule

NADPH formation:
*the high energy e- travel down the second ETC
*the e- is passed to NADP+ to make NADPH

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9
Q

non-cyclic photophosphorylation

A

involves 2 photosystems (p680,p700)
contain chlorophyll a and b
flow of electron is noncyclic (going down in one direction)
uses photolysis of water
h2o—> 2h+ +2e- +1/2 o-
end products are reduced nadph, atp, and o2

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10
Q

the reduced and oxidized form of nadp+

A

NADP+: nicotinamide adenine dinucleotide

NADPH: reduced
NADP+: oxidized

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11
Q

cyclic photophosphorylation

A

involves only photosystem 1 (at 700 nm)
end product is atp only

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12
Q

where does the production of ATP take place in photosynthesis?

A

thylakoid membrane

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13
Q

a summary of chemical processes of photosynthesis

A

h2o——light reaction (grana)——> O2
-make atp & NADPH
CO2—–calvin cycle (stroma)—–>sugars
-make adp+p & NADP+

calcin cycle is also called dark reaction bc it does not depend on light

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14
Q

what is light-independent reaction

A

aka calvin cycle
-takes place in the stroma of the chloroplast
-no light required but need end products (reduced NADPH & ATP)
-need an enzyme RUBISCO (ribulose diphosphate carboxylase) and carbon dioxide

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15
Q

What is the calvin cycle called now? + what happens

A

carbon reactions

CO2—–free energy of cleavage of P bonds of ATP + reducing power of NADPH—-> carbohydrate

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16
Q

where are the enzymes and intermediates of the calvin cycle located

A

chloroplast stroma

17
Q

what is the function of RuBP carboxylase?

A

catalyzes CO2 fixation

ribulose-1,5-biphosphate+ CO2—-RuBP—–> 2 3-phosphoglycerate

also known as RuBP carboxylase/oxygenase
most abundant enzyme on earth

18
Q

the process of calvin cycle

A

step 1: carbon fixation
3 CO2 —–rubisco—–> 3 PGA

step 2: energy consumption and redox
3 PGA —- (6ATP—> ADP+P) (6 NADPH—> 6 NADP+)—-> 2 G3P

step 3: release of one molecule of G3P

step 4: regeneration of RuBP
G3P —–(3 ADP—-> 3 ATP)—–> RuBP

19
Q

postulated mechanism of RuBP carboxylase

A

ribulose-1, 5-biphosphate—-out an H+——> enediolate intermediate——add CO2——> b-leto intermediate——-add H2O——> 3-phosphoglycerate (2)

20
Q

Carbon count in the calvin cycle

A

(3) 5-C ribulose-1, 5-bisP (a total of 15 C) are
-carboxylated (3)
-cleaved
-phosphorylated
-reduced
-dephosphorylated
==>
(6) 3-C glyceraldehyde-3-P (a total of 18 C)
-1 exists as product
5 are recycled back into (3) 5-C ribulose 1, 5-biphosphate

21
Q

factors that affect photosynthesis

A

rate=rate of oxygen production per unit mass of green plant tissue/ per unit weight of total chlorophyll

  1. light intensity (too much light damage the process-limitation)
  2. wavelength (green light not effective)
  3. carbon dioxide concentration
  4. temperature
  5. water supply (not enough)
  6. chlorophyll concentration
  7. pollution