Exam 3 Streptococcus sp. Flashcards
(51 cards)
What is the catalase test
the most important test to differentiate Gram positive Cocci: Staph from Strep
What is indicative of a positive catalase test and a negative catalase test
positive=bubbles
negative=no bubbles
What are the results for a catalase of streptococcus and enterococcus species
they are catalase negative
What are the characteristics of streptococcus and enterococcus species
they are gram positive cocci (in pains and chains) and have varying hemolytic patterns such as gamma, alpha, and beta
What is streptococcus’ drug of choice
penicillin, works by inhibiting bacterial cell wall synthesis
What are latex antigen detection
commercially available kits that allow for the rapid detection of the Lancefield classification in B-hemolytic streps
Where are streptococcus normally found
in the pharynx, mouth, lower Gi tract, and vagina
Streptococcus can cause life-threatening infections when identified in sterile sites such as
blood, csf, other body fluids (pleural, pericardial, joint, and vitreous), bone, and vascular tissue
What is the Lancefield classification of strep pyogenes
group A streptococcus
When Strep A is plated on SBA, what type of hemolysis can be seen
a large well-defined area of b-hemolysis
Sensitivity to bacitracin is
‘A’ disk positive
Resistance to bacitracin is
‘A’ disk negative
Is Strep A sensitive or resistant to bacitracin
Strep A is sensitive to bacitracin making it ‘A’ disk positive
Is group A strep pyr+ or -
pyr +
Which proteins give group A strep a virulence factor
M proteins blocks the binding of C3b (opsonization), and F protein both help with the adherence and invasion of the epithelial cells
What is Streptolysin O
streptolysin O is a hemolysin virulence factor of Group A strep that destroys WBCs, platelets, RBCs, and other tissues (oxygen labile- destroyed by oxygen)
How is Streptolysin O detected on SBA
to detect streptolysin O SBA should be stabbed to force some of the organism to grow in a reduced oxygen content
What is Streptolysin S
streptolysin S is another hemolysin virulence factor of Group A strep that can lyse RBCs and WBCs (oxygen stabile-can grow in the presence of oxygen)
What are some other virulence factors of Group A strep
hyaluronidase- a spreading factor that causes the breakdown of connective tissue
Streptokinase- lysis fibrin clots
Streptococcal pyrogenic exotoxins (SPEs)- causes a red spreading rash and acts as superantigens (SPE A,B,C, and F exotoxins)
What are infections caused by Group A strep
pharyngitis (strep throat THE MOST COMMON CAUSED BY A STREP), pyoderma (skin infections), sepsis (blood infections), and necrotizing fasciitis (streptococcal gangrene)
What are some pyoderma infections
impetigo (in very young children), erysipelas (spreading red rash), and cellulitis (deep invasion of GAS leading to necrosis and gangrene)
What are some complications that can happen post Group A infection
Rheumatic fever (occurs after pharyngitis), scarlet fever, and acute glomerulonephritis
What is the Lancefield classification of streptococcus agalactiae
Group B strep
What are some virulence factors of group B strep
capsule, hemolysin, CAMP factor