Exam 4 Flashcards by Kyle Snyder

Q

Mass Spectrometry is a ________ technique.

A

Qualitative and Quantitative

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Q

Classify the following characteristics as those describing an Anode or a Cathode.

Positive Pole

A

Anode

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Q

Classify the following characteristics as those describing an Anode or a Cathode.

Negative Pole

A

Cathode

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Q

Classify the following characteristics as those describing an Anode or a Cathode.

Oxidation Reaction

A

Anode

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Q

Classify the following characteristics as those describing an Anode or a Cathode.

Reduction Reaction

A

Cathode

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Q

Classify the following characteristics as those describing an Anode or a Cathode.

Loss of Electrons

A

Anode

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Q

Classify the following characteristics as those describing an Anode or a Cathode.

Gain of Electrons

A

Cathode

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Q

Match the following instrumentation principles involving light energy with their descriptor.

A technique used to determine the concentration of a substance by measuring the variation in its color intensity without taking the wavelength of light into account

A

Photometry

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Q

Match the following instrumentation principles involving light energy with their descriptor.

The measurement of light at a selected wavelength, typically in the ranges of UV, visible, and infrared light

A

Spectrophotometry

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Q

Match the following instrumentation principles involving light energy with their descriptor.

The measurement of light reflected from the surface of a colorimetric reaction in order to determine the amount of unknown colored product

A

Reflectance Spectrophotometry

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Q

Match the following instrumentation principles involving light energy with their descriptor.

A type of spectrophotometry which uses a beam of light, typically UV light, to excite electrons in molecules causing them to emit light of a different wavelength

A

Fluorescent Spectrophotometry

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Q

Match the following instrumentation principles involving light energy with their descriptor.

An instrumentation technique based on detection of the portion of light scattered by the particles in a solution toward a detector not in the direct path of the transmitted light

A

Nephelometry

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Q

Match the following instrumentation principles involving light energy with their descriptor.

An instrumentation technique that measures the reduction of light transmitted through a homogeneous solution due to the light’s scattering

A

Turbidimetry

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Q

Match the following instrumentation principles involving light energy with their descriptor.

The measurement of light emission via chemiluminescence, bioluminescence, and electrochemiluminescence

A

Luminometry

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Q

A spectrophotometric ‘Blank’ is composed of:

A

All of the reagents used in the procedure without the sample

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Q

The color of light seen by the naked eye is _________ . All other wavelengths are _________.

A

Transmitted, Absorbed

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Q

Visible light constitutes approximately which wavelength:

A

380 – 700 nanometers

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Q

An Ion-Selective Electrode (ISE) is permeable to which of the following:

A

Membranes selectively permeable to certain ions ( H+,K+,Na+,Mg2+)

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Q

Mass Spectrometry separates molecules based on their:

A

Mass-to-Charge Ratio (m/z)

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Q

Select the correct relationship between frequency, wavelength, and energy.

A

↑ Frequency, ↓ Wavelength, ↑ Energy

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Q

Classify the following chromatographic separation mechanisms by the mechanism in which they separate analytes:

Separates analytes based on their charge using a charged stationary phase

A

Ion Exchange Chromatography

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Q

Classify the following chromatographic separation mechanisms by the mechanism in which they separate analytes:

Separates analytes based on the differential distribution of solutes between two immiscible liquids

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A

Partition Chromatography

Q

Classify the following chromatographic separation mechanisms by the mechanism in which they separate analytes:

Separates analytes by adsorption and desorption of solutes at the surface of a solid particle

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A

Adsorption Chromatography

Q

Classify the following chromatographic separation mechanisms by the mechanism in which they separate analytes:

Separates analytes via liquid chromatography using biological interaction (ie. enzyme binding a substrate, hormone with receptor, antibody with antigen, etc.)

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A

Affinity Chromatography

Classify the following chromatographic separation mechanisms by the mechanism in which they separate analytes: Separate analytes based on their size

Size Exclusion Chromatography (gel permeation/gel filtration)

A pH electrode measures the passage of which of the following ions:

H+ Ions

The Clark Electrode is used to measure which of the following:

pO2

The relationship between wavelength and energy is mathematically depicted using __________.

Plank's Equation

Chromatography can be classified based on two phases. Which of the following is NOT a phase used to classify chromatography:

Affinity Phase

Osmolality is measured using which analytical technique:

Freezing Point Depression

Proper procedure for using a pH meter requires a calibration curve to be constructed. This is necessary in order to calculate the ______ of the line, which is used to monitor drifting/electrode deterioration.

Slope

Chromatography – particles in the mobile phase with a ______ affinity for the stationary phase migrate ______ than those with ________ affinity, which migrate ________.

higher / slower / lower / faster

Chromatography is a _______ separation technique.

Physical

Classifying a test as a Point of Care Test (POCT) refers to which of the following:

The physical location of where the test is to be performed

Match the following Electrochemistry Technique to its correct descriptor. The technique in which the electrical potential difference between two electrodes is measured under equilibrium

Potentiometry

Match the following Electrochemistry Technique to its correct descriptor. The technique that measures current flowing through an electrochemical cell and the electrochemical potential between the two electrodes while a constant external voltage is applied

Aperometry

Match the following Electrochemistry Technique to its correct descriptor. The technique that is used to measure the electrolytic conductivity of a half cell in order to monitor the progress of a chemical reaction

Conductometry

Match the following Electrochemistry Technique to its correct descriptor. The technique that measures the amount of charge, in coulombs, being passed between two electrodes at a fixed potential as a result of a chemical reaction

Coulometry

The small vessel designed to hold a liquid sample in the light path of a spectrophotometer for measurement is known as a:

Cuvette

In gas chromatography, ‘gas’ signifies the _____ phase:

Stationary

The time required for a solute to pass through a chromatographic column and reach the detector is known as the:

Retention Time

Electrochemistry involves the conversion of chemical energy into a flow of ______ within a galvanic cell.

Electrons

Which of the following is true regarding specific gravity and freezing point depression:

Specific Gravity measures the presence of solutes represented by particles of varying sizes, from small ions to larger proteins. Freezing Point Depression does not rely on particle size.

According to Beer’s law, a solution that absorbs a high amount of light would have a ______ concentration.

High

Osmolarity expresses concentrations of solutions relative to the ____ of the solvent.

Volume

The component of the spectrophotometer responsible for isolating radiant energy of a desired wavelength(s) while excluding other wavelengths is the:

Monochromator

A solution with a pH of 7.6 is considered:

Basic

The most common Indicator Electrode is known as the:

Ion Selective Electrode (ISE)

Prior to separation/categorization using a mass spectrometer, molecules must first undergo:

Ionization

Which of the following is an example of a planar form of chromatography:

Thin Layer Chromatography

Osmolality expresses concentrations of solutions relative to the ____ of the solvent.

Mass

The following chromatogram would be said to have _____.

Distinct peaks?

Light is a form of electromagnetic radiant energy that travels in:

waves