Exam III Flashcards

(49 cards)

1
Q

Properly labeled specimen collections include

A
  • Name
  • Patient identification number
  • Source of the specimen
  • Date and time of collection
  • Special instructions/comments
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2
Q

Special instructions/comments

can include

A

Precautions e.g. Smallpox, anthrax, etc.

Travel to certain endemic areas

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3
Q

Threat Agents - Contagious

A
  • Bacillus anthracis *Brucella species
  • Burkholderia pseudomallei *Francisella tularensis
  • Yersinia pestis *Blastomyces *Hystoplasma
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4
Q

Require special culture media

A
  • Bordetella pertussis *Clostridium species
  • Corynebacterium diphtheriae* *E. coli O157:H7
  • Legionella pneumophila *Vibrio cholerae
  • Yersinia enterocolitica
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5
Q

Greatest likelihood of recovering the suspected agent

A
  • Viruses during acute phase
  • Bacteria before antibiotics started
  • Parasites/Fungi no special guidance
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6
Q

Tissue or fluid specimen guideline

A

aspirate is superior to a swab for pathogen recovery

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7
Q

If Delays required for

Urine, sputum, respiratory specimens, and stool

A

Refrigerated

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8
Q

If Delays required for Cerebrospinal fluid (CSF), body fluids, and blood culture

A

Room Temperature

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9
Q

The following specimen require?
Viruses – viral transport media (VTM)
Stool – Cary-Blair media
Stool parasites – formalin and polyvinyl alcohol (PVA)

A

A tranport Media

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10
Q

Diseases that require reporting to governement agency

A
Botulism
Brucellosis
Anthrax
Tularemia
Rabies
Plague
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11
Q

Bacteremia, endocarditis, sepsis require____ specimen collection Bottles prior to _____

A

2;Antibiotics

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12
Q

(2 or more) per episode
1 “set” = 1 aerobic and 1 anaerobic bottle
Different sites/time
No more than 4 total sets within 24 hrs

A

Blood Culture

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13
Q

Pediatric Patients blood culture require

A

1 Bottle

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14
Q

Sufficient blood volume (fill line)

Adults_____ ml, Infants/children _____ml

A

20-30ml; 1-5ml

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15
Q

Growth of the same organism in repeated cultures

indicates

A

True Positive

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16
Q

Growth of different organisms in different culture bottles indicates

A

Probable contamination, bowel spillage

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17
Q

if the following are present; Coag. NEG. Staphylococci, Corynebacterium, Bacillus, Propionibacterium, indicates

A

Growth of normal skin flora – likely contamination

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18
Q

Organisms such as viridans streptococci or enterococci

A

Possible endocarditis – associated with low grade fevers

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19
Q

Nasal swabs are used to identify carriers of

A

methicillin resistant S. aureus

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20
Q

Throat swab primarily for _________ but suspect?

A

Group A streptococcal pharyngitis

Neisseria, Bordetella, Corynebacterium,

21
Q

Lower respiratory Tract specimens

A

Bronchoscopy Specimens and Sputum and Tracheal Aspirates

22
Q

specimens for diagnosis of bacterial pneumonia in ventilated patients and opporttunistic pathogens

A

Bronchoalveolar lavage fluid and protected brush

23
Q

Sputum (expectorated or induced) and tracheal aspirate for suspected

24
Q

Urinary Tract SPecimens

A

UTI, Pylonephritis, Cystitis,

25
Collection Procedure types
Clean catch- Catheter- Suprapubic Aspirate-Neonates
26
Genital Tract specimens
- Swab, Aspirate,Urine - Vaginal secretions - Endocervical and urethral specimens; Chlamydia/Gonorrhea - Vesicles-HVS - Ulcers; Syhilis
27
Reject criteria
- Improper labeling - Wrong specimen container - Leakage - Duplicate, repeat specimens - Sterile body fluid- stat
28
Panic Values
- CSF or Joint fluid - Pos, Cryptoccocal Ag detection - Pos. Acid Fast bacillus, Pos. CSF culture and TB - Isolation of E.Coli - Pathogenic Neisseria
29
To isolate virus which is best indicated in Nasopharyngeal specimen
Nasal Wash
30
Upper respiratory pathogens suspected specific of specialized media
Bordetella, corynebacterium, Neisseria
31
Genital tract infections suspected urethritis specimen
Urine or Swab
32
Stool cultures most common pathogens
Salmonella, Shigella, Campylobacter
33
Pathogen related to hospital acquired diarrhea due to ABX
Clostridium Difiicile
34
Inpatients on ABX for three days the recommendation is
No stool cultures
35
Suceptibility test drug of choice is based on
Susceptibility, cost, and availability
36
Tuberculosis specimen requirements include
1st morning sputum w/ Acid Fast Bacilli
37
Parasitology specimens ensure
stool preservative for ova and parasites
38
In tissue and subcutaneous lesions _____ are better than ______
Tissue; Swabs
39
In genital tract specimens, _________ have replaced culture
Molecular Assay
40
For a genital Tract specimen swab, aspirate or urine perform the following
- Gram Stain -Aspirated | - Wet mounts for motility
41
2nd line of Immune system defense
Innate Arm - Complement, lysozyme, interferon - Macrophages, Neutrophils, NKC (Cell mediated)
42
3rd Line of immune defense
- Cytokines, Abs | - Helper T, B Lymphocytes, plasma cells
43
Immunoglobulins that indicates current infection | (Acute or convalescent) binds second
IgG
44
Immunoglobulin that binds first during first response
IgM
45
Ummunoglobulin that binds first in second response
IgM and IgG simultaneously
46
Is a way of expressing concentration or quantitative information of a pathogen
Titer
47
Positive=Anti-HAV IgM | Positive= Anti-HAV IgG
Acute Hepatitis
48
Negative=Anti-HAV IgM | Positive= Anti-HAV IgG
Resolved Hep A
49
Negative=Anti-HAV IgM | Negative=Anti-HAV IgG
No contact w/ HAV