Exam Three

Question 1

hexokinase replaced by

Answer 1

G6Pase

Question 2

PFK replaced with

Answer 2

FBPase

Question 3

gluconeogenesis

Answer 3

fructose biphosphate and G6Pase (must be tightly controlled) happens in LIVER

Question 4

free G6Pase

Answer 4

would deplete all ATP in the cell making tight 2 enzyme cycle w/ hexokinase

Question 5

cori cycle ATP cost in liver

Answer 5

6

Question 6

cori cycle ATP cost in muscle

Answer 6

2

Question 7

CAC is ___ compared to glycolysis

Answer 7

strongly oxidative (connected to glycolysis via pyruvate DH complex)

Question 8

CAC takes place in

Answer 8

matrix (vs. glycolysis in cytoplasm)

Question 9

products of CAC

Answer 9

NADH and FADH2 (fee electrons into oxidative phosphorylation yielding ATP)

Question 10

E3

Answer 10

Pyruvate DH Complex

Question 11

succ DH

Answer 11

inner membrane/membrane bound

Question 12

irreversible steps

Answer 12

citrate synthase, pyr DH complex, isocitrate DH, alpha KGDH complex

Question 13

electron transport directly produces

Answer 13

protein gradient (drive ATP synthesis)

Question 14

malate aspartate shuttle

Answer 14

heart liver kidney

Question 15

ATP/ADP translocase

Answer 15

ATP out to cytoplasm ADP come in (antiport)

Question 16

coupling

Answer 16

at rest rate of O2 goes down bc ATP is high

Question 17

uncoupling

Answer 17

abnormal state can be induced by various things(UCPI, 2,4 DNP) symptoms: hot, rapid breathing, feel weak, burning tops of fuel but not making ATP bc ATP synthesis is uncoupled from O2 use

Question 18

complex I inhibitor

Answer 18

retenone, amytal

Question 19

complex III inhibitor

Answer 19

antimycin A

Question 20

complex IV inhibitor

Answer 20

cyanide, azide, carbon monoxide

Question 21

PPP makes

Answer 21

NADPH and ribose

Question 22

30 carbon rxn

Answer 22

want sugar but no NADPH: non ox branch

Question 23

fixed ratio of 2 NADPH for every Ribose 5P

Answer 23

oxidative branch

Question 24

only NADPH and no ribose 5P

Answer 24

part of oxidative and non ox (resembles CAC)

Question 25

glutathione

Answer 25

"redox" buffer to protect cells against oxidation

Question 26

Wenicke Korsakoff syndrome

Answer 26

lack of thiamine can't saturate enzymes mut in transketolase

Question 27

Calvin cycle

Answer 27

rxn from gluceoneo and PPP

Question 28

"hitting the wall"

Answer 28

depleting glycogen (need 8 pieces of bread, big piece of cake, or banana split)

Question 29

"a" form of glycogen phosphorylase

Answer 29

added phosphates, normally "on", LIVER

Question 30

"b" form of glycogen phosphorylase

Answer 30

no phosphates, "off" (can be turned on allosterically by AMP low E charge) MUSCLE (turned on during exercise)

Question 31

F-2,6-BP signals

Answer 31

plenty of glucose

Question 32

pyruvate carboxylase works perfectly in absence of

Answer 32

NAD+

Question 33

pyruvate DH complex - FAD is attached to

Answer 33

E3

Question 34

2 enzymes with FeS cluster

Answer 34

succinate DH, aconitase

Question 35

not inhibited by ATP and NADH

Answer 35

malate DH

Question 36

C3 of pyruvate labelled one turn of CAC

Answer 36

C2 and C3

Question 37

isocitrate lyase is important in

Answer 37

glyoxylate cycle

Question 38

cyto c is located in

Answer 38

intermemb space

Question 39

pamaquine induced hemolytic anemia

Answer 39

G6PDH deficiency, RBC membrane cross links get brittle

Question 40

calvin cycle

Answer 40

PPP + gluconeogenesis

Question 41

transkeltolase

Answer 41

covalent attachment of substrate to thiamine phosphate

Question 42

F1

Answer 42

contains catalytic sites for ATP synth, contains alpha beta gamma subunits, mostly matrix

Question 43

F0

Answer 43

proton channel, spans inter mito membrane, contains a b c subunits

Question 44

TPP at active site

Answer 44

E1

Question 45

C1 labelled 1 turn of CAC

Answer 45

none lost as CO2

Question 46

substrate level phosphorylation in CAC catalyzed by

Answer 46

succinyl CoA synthase

Question 47

ubiquinone

Answer 47

linked btwn electron transport and proton pumping moving H+ across mito membrane

Question 48

"sugar can make you fat but not sweet" not true for

Answer 48

glyoxalate cycle

Question 49

glycerol phosphate shuttle

Answer 49

glycerol phosphate + DHAP

Question 50

C2 labelled after 1 turn of CAC

Answer 50

C4 is labelled

Question 51

succ DH does not catalyze

Answer 51

oxidative decarboxylation

Question 52

gamme subunit of Racker's Knob

Answer 52

camshaft

Question 53

ox of 1 mole of Acetyl CoA produces

Answer 53

10 ~P

Question 54

cofactor of transketolase

Answer 54

TPP

Question 55

ketone bodies do NOT include

Answer 55

alpha KG and OAA

Question 56

anaplerotic rxns in mammals

Answer 56

convert pyruvate in OAA

Question 57

cori cycle

Answer 57

responsible for lactate and glucose in blood

Question 58

ATP formed when electrons from FADH2 are transported to O2 via MET

Answer 58

1.5

Question 59

transaldolase rxn

Answer 59

Sedo7P + G3P = E4P + F6P

Question 60

transketolase

Answer 60

Eryth4P + Xylulose 5P = Fru6P + Gly3P