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MCB 101 Lab > Exercise 3_ Morphology of Filamentous Fungi > Flashcards

Exercise 3_ Morphology of Filamentous Fungi Flashcards

(105 cards)

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1
Q

main character for exer 3

A

filamentous fungi

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2
Q

optimal temperature on which molds will grow

A

at 25 °C

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3
Q

25 °C is the optimal temperature for growth of what? yeast or mold

A

mold

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4
Q

species of molds where dichotomous branching is present

A

Allomyces Galactomyces geotrichum

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5
Q

Allomyces and Galactomyces geotrichum are representative species with

A

dichotomous branching

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6
Q

this could either be branched or unbranched

A

hyphae

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7
Q

2 forms of hyphae

A

branched or unbranched

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8
Q

Collective form of hyphae

A

mycelia

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9
Q

Two forms of molds

A

filamentous or multicellular fungi

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10
Q

Forms of mycelium

A

septated or non septated

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11
Q

defining feature between individual cells in the mycelium when it is either septated or non septated

A

crosswalls (septa)

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12
Q

allows the exchange of cytoplasm or organelles throughout the hyphae

A

crosswalls

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13
Q

what happens in the crosswalls between the individual cells in the mycelium

A

allow exchange of cytoplasm or organelles

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14
Q

Location where the branches of septated fungi are situated

A

immediately behind a septum

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15
Q

In septated hyphae, this could be seen immediately at the back of the septum

A

branches

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16
Q

this form of hypae tends to be continuous along the whole length of the filament

A

non septated hypha

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17
Q

two results of the non septation of hypa

A

coenocytic multinucleated

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18
Q

what imparts color, texture and overall appearance to the mold colony?

A

mycelium

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19
Q

Since the hypha is a structural unit, the mycelium imparts what to the mold colony?

A

color texture overall appearance

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20
Q

When melanin pigments are present, what is the resulting color of the colonies?

A

with dark pigmentation

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21
Q

location where melanin pigments are situated

A

cell walls of molds

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22
Q

this are molds with melanin pigments in their cell wall

A

dematiaceous

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23
Q

dematiaceous are molds with what?

A

melanin pigments in their cell wall

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24
Q

with the absence of pigments in their cell wall it is described as

A

hyaline

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25
hyaline coincides with molds that
does not produce pigments in their cell walls
26
this means transparent or translucent
hyaline
27
hyaline is observed as
transparent or translucent
28
mycelial differentiation
submerged aerial fertile
29
type mycelial differentiation that penetrate and anchor the thalli in the substrate and absorb the nutrients
submerged mycelia
30
functions of submerged mycelia
anchor the thalli in the substrate absorb the nutrients
31
mycelia that grow above the substra
aerial mycelia
32
where aerial mycelia is situated
above the substrate
33
reproductive structures that fertile mycelia bear
conidia or sporangia
34
type of mycelia that harbors conidia or sporangia
fertile mycelia
35
the asexual spores in molds
sporangiospores conidia
36
sporangiospores and conidia are what type of spores? asexual or sexual
asexual
37
The sac where sporangipores are formed
sporangium
38
The sac called sporangium houses what spores?
sporangiospores
39
stalks where the sporangia is attached
sporangiophores
40
the sporangiophores stalks are attached with?
sporangia
41
specialized hyphae
conidiophores
42
conidiophores are _____ ____ where conidia is formed
specialized hyphae
43
what is formed on specialized hyphae called conidiophores?
conidia
44
small conidia
microconidia
45
large multicellular conidia
macroconidia
46
types of conidia (4)
phialospores blastoconidia arthrospores chlamydospores
47
phialospores, blastoconidia, arthrospores, chlamydospores are types of?
conidia
48
vase-shaped cells
phialides
49
conidia are produced by vase-shaped cells called phialides
phialospores
50
where does the conidia of phialospores produced?
phialides (vase-shaped cells)
51
the conidia of blastoconidia is produced from
budding of pre existing conidia
52
conidia formed by separation from preexisting hyphal cells
arthrospores
53
h does the conidia of arthropores are formed
by separation from preexisting hyphal cells
54
characterized by spores that are large, thick-walled, round or irregular structures formed within or on the ends of a hypha
chlamydospores
55
conidia of chlamydospores are formed where?
formed within or on the ends of the hypha
56
conidia are produced from budding of pre existing conidia
blastoconidia
57
sexual reproduction in molds involves the union of what
compatible nuclei
58
the union of compatible nuclei pertains to the
sexual reproduction in molds
59
the product of mating between 2 different organisms or hyphae
sexual spores
60
sexual spores of molds
oospores ascospores zygospores basidiospores
61
oospores, ascospores, zygospores, basidiospores are what type of spores
sexual spores
62
produced when male gametes enter a large spherical cell called oogonium and fertilized the egg within
oospore
63
oospores are produced produced when male gametes enter a large spherical cell called ______ and fertilized the egg within
oogonium
64
formed by union of two hyphal protrusions from neighboring hyphae of two different strains
zygospores
65
zygospores are formed by the union of wha
two hyphal protrusions from neighboring hyphae of two different strains
66
produced in enclosures which may be oval sacs or elongated tubes called asci
ascospores
67
ascospores are formed inside of what structure
asci
68
produced externally on club-shaped bodies called basidia
basidiospores
69
basidiospores are formed on
club-shaped bodies called basidia
70
culture media utilized for this activity
Czapek Dox Agar (CDA) Potato dextrose agar (PDA)
71
Mold specimens utilized for this exercise
Fusarium Geotrichum Penicillium Rhizophus Trichoderma
72
the method used to characterized selected filamentous fungi morphologically
slide culture method
73
Slide culture method is utilized to characterize molds based on its
morphology
74
What is prepared to be placed inside the petri plate before sterilization of the slide culture set up
2 toothpicks 1 glass slide 1 cover slip circular paper towel/filter paper
75
Incubation period for the growth of mold colony
1-7 days
76
During the sequential point inoculation of molds, which medium was inoculated first?
CDA to PDA
77
Incubation period for slide culture set up
2-7 days
78
media used for slide culture set up
PDA
79
amount of sterile water to be used in the slide culture set up
until the filter or paper towel is properly mositened
80
do you invert the slide culture set up?
No
81
before mounting the glass slide from the slide culture set up, what is the very f1st thing to do?
wipe the moisture at the bottom part of the slide
82
why is it that we invert the plate during the inoculation of molds?
- to insure that only the right amount of spores will be inoculated - to minimize spore dispersal
83
approximate measurement of the agar blocks to be utilized in slide culture set up
1cm
84
alternative method that can be used to prepare slide cultures
drop method
85
mounting medium of the wet mount of the slide culture
lactophenol
86
how many drops of lactophenol is utilized?
1-2 drops
87
Replacement of lactophenol as mounting medium of permanent mount is desired
canada balsam
88
canada balsam can replace _____ as mounting medium for slide culture observation
lactophenol
89
stolon and rhizoids are what type of structures
vegetative structures
90
specialized vegetative structures that can be observed from slide culture set
stolon rhizoids etc
91
type of conidia present in Geotrichum
arthrospore
92
arthrospore can be present in what mold specimen we used?
Geotrichum
93
type of conidia present in Penicillium
phialospores
94
phialospores can be present in what mold specimen we used?
Penicillium
95
conidia present in Fusarium
macro and microconidia
96
macro and microconidia can be present in what mold specimen we used?
Fusarium
97
Sporangiospores can be present in what mold specimen we used?
Rhizophus
98
type of spores present in Rhizopus
sporangiospores
99
what other structures are seen in the Rhizopus specimen we used?
stolon rhizoid
100
stolon and rhizoid are present in what mold specimen we used?
Rhizopus
101
branched conidiophores can be present in what mold specimen we used?
Trichoderma
102
trichoderma is observed with ____ ____ under the microscope
branched conidiophores
103
staining agent that is present in lactophenol
cotton blue
104
the component of lactophenol that preserves the fungal structure
lactic acid
105
function of phenol as a component of a mounting medium
serves as disinfectant that kills other living organisms

MCB 101 Lab (7 decks)

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