Experiment 3: Chromatographic Separation of Philippine Fishes Flashcards
(15 cards)
What is an important biophysical technique that enables the separation, identification, and purification of the components of a mixture for qualitative and quantitative analysis?
Chromatography
What are the 4 separation methods in chromatography?
- Adsorption Chromatography
- Partition Chromatography
- Exclusion Chromatography
- Ion Exchange Chromatography
What kind of separation method was used for TLC?
Adsorption Chromatography
What are the 2 mobile phases in chromatography?
- Liquid Chromatography
- Gas Chromatography
What mobile phase was used in TLC?
Liquid chromatography
What are the 3 stationary phases in chromatography?
- Solid Chromatography
- Liquid Chromatography
- Gas Chromatography
What stationary phase was used in TLC?
Solid chromatography
What is a chromatographic technique used to separate the components of a mixture using a thin stationary phase supported by an inert backing?
Thin Layer Chromatography (TLC)
What is the separation based on?
- polarity
- interactions of the sample, adsorbent, and solvent molecules
What adsorbent does the mobile phase move over?
Silica or alumina
What does the use of TLC suggest about the unknowns?
The unknowns have high polarity
What is the purpose of the ninhydrin test?
Visualizing agent that detects alpha amino acid
What is the ratio of the distance travelled by the solute to the distance travelled by the solvent?
Retention Factor (Rf)
What separation method was used in paper chromatography?
Partition Chromatography
Give the 9 essential amino acids
- Histidine
- Isoleucine
- Leucine
- Lysine
- Methionine
- Phenylalanine
- Threonine
- Tryptophan
- Valine
