extra

Question 1

lineweaver burke – what is x and y intercept and slope

Answer 1

x intercept :-1/km; y intercept: 1/v; slope: k/v

Question 2

as substrate increases __ increases

Answer 2

v max

Question 3

as enzyme increases, __ increases

Answer 3

v max

Question 4

what is km

Answer 4

the substrate at 1/2 max velocity

Question 5

2 models for cooperativity

Answer 5

sequential - one subunit changed at a time; concerted – all subunits activated at once

Question 6

competitive inhibitino

Answer 6

binds at substrate binding site and blocks it, increased substrate can overcome; increases km and no effect on vmax

Question 7

non competitive inhibition

Answer 7

binds to separate site on the enzyme and keep substrate from binding; lowers the amount of active enzyme; v max decreases but no change in km

Question 8

uncompetitive inhibitor

Answer 8

binds to the ES complex and prevents product formation; both vmax and km decrease

Question 9

allosteric activator

Answer 9

stabilizes the R conformation, km decreases

Question 10

allosteric inhibitor

Answer 10

stabilizes the T state; Km increases

Question 11

in DNA replication, how is the parent strand read? how is the replication strand made?

Answer 11

parent strand read 3 to 5 so the replication (leading and lagging) are made 5 to 3

Question 12

how does heme and heme regulated inhibitor kinase work

Answer 12

heme regulated inhibitor kinase (HRI kinase) inactivates eif2 from making globin. heme comes in and inactivates the kinase, thus heme allows eif2 to be active and produce globin.

Question 13

how does non disjunction in anaphase 1 work? result in anaphase 2?

Answer 13

Non-disjunction in anaphase (I or II) can lead to trisomy. In anaphase I, failure of homologs to separate can lead to two gametes with trisomy, and two with monosomy. In anaphase II, failure of homologs to separate can lead to one gamete with monosomy, one with trisomy, and two normal gametes.