Extra Practice!! Flashcards
(13 cards)
To count apoptotic nuclei, use __________ staining and a __________ microscope.
Syto24; fluorescent
To quantify a specific protein, we use a __________ and a __________ antibody
Western blot; specific
A competitive ELISA is also known as __________ ELISA.
inhibition
What ELISA would you use to measure IL-1β concentration in supernatant? List basic steps
Sandwich ELISA; Coat the well with antibody, WASHm add antigen to be measured, add enzyme-linked detection antibody, WASH and add substrate, measure color
- The only antibody that can cross the placenta is __________.
IgG
A high neutrophil count could indicate __________.
bacterial infection
A high eosinophil count could indicate __________.
parasititic infection
__________ are white blood cells that make up 2–8% of leukocytes.
monocytes
What’s the purpose of washing cells before using CyQuant?
Dead cells can contribute free DNA, leading to falsely high fluorescence
Direct Elisa Steps
1.Antigen coated well
2. wash
3. add enzyme conjugated antibody to be measured
4. wash
5. add substrate and measure color
Antigen is immobilized on surface
Enzyme linked antibody, specific to the antigen
Indirect ELISA steps
- antigen coated well
- wash
- add antibody to be measured
- wash
- add enzyme conjugated secondary antibody
- wash
- add substrate and measure color
used for measuring antibodies in a serum
sandwich ELISA steps
- antibody coated well
- wash
- add antigen to be measured
- wash
- add enzyme conjugated secondary antibody
- wash
- add substrate and measure color
used for measuring antigen levels in a solution
competitve ELISA steps
- incubate antibody with antigen to be measured
- add ag-ab mixutre to antigen coated well
- wash
- add enzyme conjugated secondary antibody
- wash
- add substrate and measure color
used for detecting small antigens that cannot be bound by 2 different antibodies–no sandwich
