extracting dna from fruit Flashcards
(6 cards)
1
Q
why is the fruit crushed and filtered?
A
break apart cell walls and remove insoluble bits
2
Q
why is washing-up liquid used?
A
breaks down membranes of the cell and nucleus
3
Q
what does salt do?
A
precipitate the dna from the solution
4
Q
why do we need to cool the mixture?
A
slows down enzymes that would digest the dna
5
Q
what does cold ethanol do?
A
causes dna to clump together
6
Q
why can’t room temp. ethanol be used?
A
doesn’t make dna visible
