F11

Question 1

If the acid and gas are formed in the multiple-tube fermentation technique, the test is ___
Positive
Continued
Negative
Discarded

Answer 1

Positive

Question 2

Which of the following is the standard for drinking water?
Clear, Colorless, free from objectionable taste and odor
Clear, Flavourful, Odorless
Both Choices are correct
None of the choices are correct

Answer 2

Clear, Colorless, free from objectionable taste and odor

Question 3

Which of the following is produced by Escherichia coli?
Orthonitrophenyl-B-D-Galactopyranoside (ONPG)
4-Methylumbelliferyl-B-D-Glucuronide (MUG)
B-Glucuronidase
B-D-Galactosidase

Answer 3

B-Glucuronidase

Question 4

How much of the diluted sample is needed for Spread Plate Method?
0.1-0.5 mL
0.1-2.0 mL
0.3-0.5 mL
0.5-3.0 mL

Answer 4

0.1-2.0 mL

Question 5

Which of the following medium is used for total coliform in the confirmed phase of the Multiple Tube Fermentation Technique?
BGLB
EC Medium
LTB
TSA

Answer 5

BGLB

Question 6

A Laboratory analyst performed the confirmed test and obtained the following results; Gas Formation inside the Durham Tube, Presence of Effervescence and Turbidity. Which would be the next course of action after observing these results?
Report as Negative for Coliforms
Report that water is safe to drink
Perform the completed test to detect the presence of fecal coliform
Repeat the procedure because air is present inside the Durham tube

Answer 6

Perform the completed test to detect the presence of fecal coliform

Question 7

All of the following are methods under the Heterotrophic plate count, EXCEPT?
1- Pour plate method
2- Quantitray method
3-Enzyme-substrate method
4- Spread plate method
5- Membrane filter method
1 and 3
1 and 2
2 and 3
1,2,3
3,4,5

Answer 7

2 and 3

Question 8

How often should residential buildings with 700 occupancies be subjected to microbiology exams of drinking water?
1 sample every week
1 sample monthly
1 sample every other month
1 sample annually

Answer 8

1 sample monthly

Question 9

What is the standard value for total coliform in Membrane Filter Technique?
<1.1 MPN coliform colonies/100 mL
<1 MPN/100 mL
<1 total coliform colonies/100 mL
<500 est. CFU/mL

Answer 9

<1 total coliform colonies/100 mL

Question 10

Which of the following medium is used for the presumptive phase in the Multiple Tube Fermentation Technique?
Lauryl Tryptose Broth
Eosin-Methylene Blue Agar
Escherichia coli Medium
Brilliant Green Lactose Bile Broth

Answer 10

Lauryl Tryptose Broth

Question 11

Which of the following techniques is used for bacterial monitoring in the pharmaceutical industry?
Membrane Filter Technique
Multiple Tube Fermentation Technique
Enzyme substrate coliform test
Heterotrophic Plate Count

Answer 11

Membrane Filter Technique

Question 12

Which of the following is preferred for low-count water?
Pour Plate Method
Spread Plate Method
Membrane Filter Method
None of the choices are correct

Answer 12

Membrane Filter Method

Question 13

Which of the following parameters for bacteriological analysis would be able to specifically detect the presence of pathogenic E. coli in water?
Total coliform count
Thermotolerant coliform
Heterotrophic plate count
All of the above

Answer 13

Heterotrophic plate count

Question 14

Among the standard methods for Total coliform count and Thermotolerant coliform count, which utilizes hydrolyzable substrate for detection of coliform and E. coli?
Multiple tube fermentation technique
Enzyme-substrate technique
Membrane filter technique
Only A and B is correct
Only B and C is correct

Answer 14

Enzyme-substrate technique

Question 15

MTFT method, which media would you use in the Confirmed test and what would be the positive result?
a. Lauryl tryptose broth-growth with bubbles and turbidity
b. EMB-colony growth with greenish metallic sheen
c. BGLB-growth with bubbles and turbidity
d. EC medium - growth with bubbles and turbidity
If only A and B is correct
If only C and D is correct
If all is correct
If none is correct

Answer 15

If none is correct

Question 16

A medical technologist performed the presumptive test and the broth tubes shows a negative result after the first 24 hours. Which would be the next course of action?
Report as Negative for Coliforms.
Proceed to the Confirmed Phase.
Re-incubate for another 24 hours.
Proceed to the Completed Phase

Answer 16

Re-incubate for another 24 hours.

Question 17

Examine both statements below and determine which is TRUE and which is False. 1. The West Avenue Medical center is the National Reference Laboratory for Bacteriologic Analysis ofwater.
II. The Philippine National Standards for Drinking Water is embodied in the DOH A.O. #17 series of 2010.
Both statements are TRUE.
Both statements are FALSE.
First statement is TRUE; Second statement is FALSE
First statement is FALSE; Second statement is TRUE

Answer 17

Both statements are FALSE.

Question 18

Which of the following statement is/are correct regarding coliform bacteria? i. It is gram-negative
ii. It is rod-shaped bacteria iii. It is a harmless aerobic microorganism
i,ii,iii
ii,iii
i,iii
i,ii

Answer 18

i,ii,iii

Question 19

A medical technologist performs a heterotrophic plate count in a water sample. Using a glass petri dish he/she was able to count > 100 colonies/cm2, which of the following would be the proper reporting of the result?
Report as >5,700 est. CFU/mL
Report as >6,500 est. CFU/mL
(count) est. CFU/mL
(count) CFU/mL
None of the above

Answer 19

None of the above

Question 20

Examine both statements below and determine which is TRUE and which is False. 1. Total Coliform Bacteria detects the enzyme B-D-Galactosidase which is produced by coliform bacteria. II. Fecal Coliform Bacteria detects enzyme B-Glucuronidase which is produced by Escherichia coli.
Both statements are TRUE.
Both statements are FALSE..
First statement is TRUE; Second statement is FALSE
First statement is FALSE; Second statement is TRUE

Answer 20

Both statements are TRUE.