Factors Affecting Microbial Growth and Food Handling

Question 1

Common Bacteria

Answer 1

E. coli (Escherichia Coli), Salmonella, Norovirus, Listeria

Question 2

How many known pathogens are there?

Answer 2

31 but there are millions out there we just can’t culture them

Question 3

Food Spoilage Bacteria

Answer 3

Pediococcus, Lactococcus, Acinetobacter

Question 4

Are viruses alive?

Answer 4

No, viruses are dead because they are particles

Question 5

Bacteria that can hurt you

Answer 5

Staphylococcus, Clostridium botulinum, Prion disease, Campylobacter

Question 6

Where is Staphylococcus found?

Answer 6

High salt, raw food, raw milk

Question 7

Is Staphylococcus aerobic or anerobic?

Answer 7

Anerobic

Question 8

What is it called when Staphylococcus infects someone?

Answer 8

Intoxication

Question 9

Where is Clostridium botulinum found?

Answer 9

Canned food

Question 10

Is Clostridium botulinum aerobic or anerobic?

Answer 10

Anerobic

Question 11

What is meant by Prion disease?

Answer 11

Creutzfeldt-Jakob disease/Bovine Spongiform Encephalopathy (Mad Cow Disease)

Question 12

Where is Prion Disease found?

Answer 12

Infected meat

Question 13

Is Prion Disease anerobic or aerobic?

Answer 13

Anerobic

Question 14

Where is Campylobacter found?

Answer 14

Raw or undercooked poultry

Question 15

Is Campylobacter aerobic or anerobic?

Answer 15

TRICK QUESTION! It is Microaerobic meaning it need very small amounts of oxygen.

Question 16

What is a self limiting disease?

Answer 16

A disease that can resolve itself within a few days, doesn’t require medical intervention

Question 17

What are some self limiting diseases?

Answer 17

Norovirus, Campylobacter, Salmonella

Question 18

What does YOPI stand for?

Answer 18

Y: Young
O: Old
P: Pregnant
I: Immunocompromised

Question 19

What is YOPI used for?

Answer 19

It indicates who is at the highest risk for being infected most often

Question 20

What are the top 6 most common food poisoning symptoms?

Answer 20

1. Diarrhea
2. Vomiting
3. Nausea
4. Fever
5. Stomach Cramps
6. Dehydration

Question 21

How many layers does a gram positive bacteria cell have?

Answer 21

Three

Question 22

What are the layers of a gram positive bacterial cell?

Answer 22

Inside: Plasma membrane

Middle: Periplasmic space

Outside: Peptidoglycan

Question 23

What color does a gram positive bacterial cell stain?

Answer 23

Purple

Question 24

How many layers does a gram negative bacterial cell have?

Answer 24

Five

Question 25

What are the layers of a gram negative bacterial cell?

Answer 25

Inside: Plasma membrane Middle: Periplasmic space, Peptidoglycan, Periplasmic space Outside: Outer membrane (Lipopolysaccharide and protein)

Question 26

What color do gram negative bacterial cells stain?

Answer 26

Pink.

Question 27

What are the four types of microorganisms?

Answer 27

Protozoa, Viruses, Fungi, Bacteria

Question 28

Is protozoa eukaryotic or prokaryotic?

Answer 28

Eukaryotic

Question 29

What are the two microorganisms that are single cell organisms?

Answer 29

Protozoa and Bacteria

Question 30

What kind of microorganism is fungi? How many cells does it have?

Answer 30

Single or multi-celled organism (mold/yeast)

Question 31

How many cells do viruses have?

Answer 31

No cells, non-cellular organism and require a host to grow

Question 32

Are bacteria alive or dead?

Answer 32

Alive

Question 33

What shape are bacilli?

Answer 33

Rods

Question 34

What chape are cocci?

Answer 34

Spherical

Question 35

What shape are spirilla?

Answer 35

Sprial

Question 36

What are the types of cell grouping?

Answer 36

Pairs, clusters, and chains

Question 37

How do bacteria grow?

Answer 37

Binary Fission

Question 38

What is binary fission?

Answer 38

Mitosis but for bacteria (cells split into two new bodies)

Question 39

Do protozoa use binary fission?

Answer 39

No, they are an eukaryote so they go through mitosis.

Question 40

What are the differences in staining between gram positive and gram negative bacteria?

Answer 40

In gram-negative the bacteria is white during decolorization, whereas gram-positive is purple. In gram-negative the bacteria is pink during the counter stain with Safranin, whereas gram-positive is purple.