Facts

Question 1

Ratios of Immunoglobulins

Answer 1

IgG = 80%
IgA = 15%
IgM = 5%
IgD and igE = < 1%

Question 2

Monoclonal Gammopathies Classifications

Answer 2

Malignant - Multiple Myeloma, Waldentrom macroglobulinemia, solitary plasmacytoma, AL amyloidosis

Pre-malignant - MGUS (monoclonal gammopathy of undetermined significance), smoldering myeloma

Secondary - autoimmune disease, chronic infections, primary and secondary immune deficiency, some non lymphoid cancers

Question 3

Testing for monoclonal gammothies

Answer 3

Blood tests: CBC, Calcium, Creatinine, Serum B microglobulin, lactate dehydrogenase, SPEP, immunofixation or immunotyping, serum immunoglobulin quantification, serum free light chains

Urine: Urine protein electrophoresis,

Question 4

Materials needed for capillary electrophoresis

Answer 4

1. serum or urine sample
2. protein 6 kits (3 vials of buffer, four filters)
3. capillarys 3 instrument

Question 5

Output of electrophesis instrument

Answer 5

Electrophoretic Scan