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tagumpay Sa MARCH 2024! > Family ENTEROBACTERIACEAE > Flashcards

Family ENTEROBACTERIACEAE Flashcards

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1
Q

All ENTEROBACTERIACEAE is CATALASE POSITIVE Except:

A

S. Dysenteriae

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2
Q

All ENTEROBACTERIACEAE are OXIDASE NEGATIVE Except:

A

Plesiomnas

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3
Q

Capsular antigen; heat labile

A. K antigen
B. O antigen
C. H antigen

A

A. K antigen

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4
Q

Somatic antigen; Heat stable;
Antibodies are predominanty IgM

A. K antigen
B. O antigen
C. H antigen

A

B. O antigen

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5
Q

Located in the flagella;
Dentatured or removed by heat or alcohol;
Mainly IgG

A. K antigen
B. O antigen
C. H antigen

A

C. H antigen

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6
Q

RAPID LACTOSE FERMENTERS:

A

“EKE”
Escherichia
Klebsiella
Enterobacter

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7
Q

Late lactose fermenter:

A

” Cit Ser, Yer Haf Sa Shi is late”
Cit-robacter
Ser-ratia
Yer-sinia enterocolitica
Haf-nia
Sa-lmonella arizonae
Shi-gella sonnei

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8
Q

Non-lactose fermenter

A

Salmonella
Shigella
Proteus
Providencia
Morganella
Edwardsiella
Erwinia (plant pathogen)

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9
Q

CAUSES UTI 90%, SEPSIS, DIARRHEAL DISEASE

TSI: A/A GAS+ H2S-
IMVIC: ++–
LDC: +
ODC: +
ADH: -
COLONIES: GREEN METALLIC SHEEN ON EMB

A

E. COLI

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10
Q

MOST COMMON CAUSE OF UTI IN HUMANS

A

UROPATHOGENIC E.COLI (UPEC)

  • AEROBACTIN: CHELATES IRON
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11
Q

Cholera-like toxin

MONTEZUMA’S revenge/ turista/
Travellers diarrhea characterized
by profuse watery stool

A

ETEC/ ENTEROTOXIGENIC E.COLI

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12
Q

Shigella-like infection;
Dysentery; usually in young children living in areas of poor sanitation

Stool with RBCS, NEUTROPHILS AND MUCUS

A

EIEC: ENTEROINVASIVE E.COLI

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13
Q

Infantile diarrhea;
Non-invasive, seen in newborns and infants
Watery diarrhea with mucus but no blood

A

EPEC: ENTEROPATHOGENIC E.COLI

(EPEC inpant)*😂

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14
Q

Shiga/ Shigella-like toxin;
Most frequently asso. With certain serotypes such as E.COLIO157:H7

A

EHEC: ENTEROHEMORRHAGIC E.COLI /
VTEC: VEROTOXIN E.COLI

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15
Q

E.COLI O157:H7 on SMAC:

A

Colorless

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16
Q

Most secere manifestation of EHEC

A

HUS

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17
Q

Watery Diarrhea

A

EAEC: ENTEROAGGREGATIVE E.COLI

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18
Q

All E.coli is MUG+ EXCEPT:

A

E.COLIO157:H7

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19
Q

TSI: A/A GAS+ H2S-
IMVIC: –++
LDC: +
ODC: -
ADH: -
Nonmotile
Urease: +
COLONIES: exhibit mucoid growth, large polysaccharide
Pink mucoid on MAC

A

Klebsiella spp.

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20
Q

Friedlander’s bacillus
Encapsulated and appears as mucoid colonies that tend to string

A. K pneumoniae
B. K.rhinoscleromatis
C. K. ozaenae

A

A. K pneumoniae

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21
Q

Prurulent sinus infection
A. K pneumoniae
B. K.rhinoscleromatis
C. K. ozaenae

A

C. K. ozaenae

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22
Q

Granuloma of the nose and oropharynx
A. K pneumoniae
B. K.rhinoscleromatis
C. K. ozaenae

A

B. K.rhinoscleromatis

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23
Q

Asso. With plasmid-mediated ESBL’s

A

Klebsiella

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24
Q

IMVIC: +-++
Carry heat labile cytotoxin, that has been isolated from patients who have developed a self-limiting ANTIBIOTIC ASSO. HEMORRHAGIC COLITIS

A

K.oxytoca

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25
Infections are asso with CONTAMINATED medical devices, such as respirators; MOTILE Lactose fermenters that produce mucoid colonies TSI: A/A GAS+ H2S- IMVIC: --++
Enterobacter spp.
26
Increasingly isolated from community Acquired pyrogenic liver abscess
K1 capsular containing K.pneumoniae
27
Indole negative A. K.pneumoniae B. K.oxytoca
A. K.pneumoniae
28
Indole Positive A. K.pneumoniae B. K.oxytoca
B. K.oxytoca
29
LDC: + ODC: - ADH: - A. P.shigelloides B. E.cloaceae C. K.oxytoca, .K.pneumoniae D. E. aerogenes F. P.agglomerans
C. K.oxytoca, .K.pneumoniae
30
LDC: + ODC: + ADH: - A. P.shigelloides B. E.cloaceae C. K.oxytoca, .K.pneumoniae D. E. aerogenes F. P.agglomerans
D. E. aerogenes
31
LDC: - ODC: + ADH: + A. P.shigelloides B. E.cloaceae C. K.oxytoca, .K.pneumoniae D. E. aerogenes F. P.agglomerans
B. E.cloaceae
32
LDC: - ODC: - ADH: - A. P.shigelloides B. E.cloaceae C. K.oxytoca, .K.pneumoniae D. E. aerogenes F. P.agglomerans
F. P.agglomerans
33
LDC: + ODC: + ADH: + A. P.shigelloides B. E.cloaceae C. K.oxytoca, .K.pneumoniae D. E. aerogenes F. P.agglomerans
A. P.shigelloides
34
Gained notoriety with a nationwide outbreak of septicemia resulting from contaminated IV fluid. A. P.shigelloides B. E.cloaceae C. K.oxytoca, .K.pneumoniae D. E. aerogenes F. P.agglomerans
F. P.agglomerans * Pantoea agglomerans HG XII, produce yellow pigment, primarily a plant pathogen
35
Produces Pink to Red colonies due to prod. Of Prodigiosin after incubation at 25°C ONPG, DNAse, gelatinase, lipase: + MDR / resistant to many antimicrobials IMVIC: --++ TSIA: K/A GAS+ H2S-
Serratia spp.
36
Most clinically significant Serratia spp. Infection of urinary and respiratory tract, bacterimic outbreaks, septic arthritis A. S.odorifera B. S. marcescens C. S.plymuthica
B. S. marcescens
37
Has a musty and pugent odor or a ROTTEN POTATO-LIKE odor A. S.odorifera B. S. marcescens C. S.plymuthica
A. S.odorifera
38
Osteomyelitis was found following a motorcycle accident A. S.odorifera B. S. marcescens C. S.plymuthica
C. S.plymuthica
39
Asso with renal stone formation Are motile, Non-LF capable of deaminating phenylalanine; CULTURE MEDIA: Swarming appearance ODOR: CHOCOLATE CAKE/ BURNT CHOCOLATE
Proteus spp. * Renal stone is called staghorn / struvite stone
40
OX-2, OX-19 ACQUIRED B PHENOMENON A. P.vulgaris B. P.mirabilis
A. P.vulgaris *Also E. coli 086
41
OX-k ACQUIRED A PHENOMENON A. P.vulgaris B. P.mirabilis
B. P.mirabilis
42
IMVIC: -+vv TSIA: K/A, GAS+, H2S+ LIA R/A Indole - A. P.vulgaris B. P.mirabilis
B. P.mirabilis
43
IMVIC: ++-v TSIA: K/A, GAS+/-, H2S+ LIA R/A Indole + A. P.vulgaris B. P.mirabilis
A. P.vulgaris
44
ONPG+ LDC - A. CITROBACTER SPP B. SALMONELLA SPP
A. CITROBACTER SPP
45
Asso. with infections disease acquired in hospital setting, UTI, pneumonia, inta-abdominal abscesses Asso with endocarditis in IV drug abusers 80% H2S+ 50% NON-LF 70% hydrolyze urea LDC: NEGATIVE A. C.freundii B. C.koseri C. Salmonella
A. C.freundii
46
Cause of nursery outbreaks of neonatal meningitis and brain abscess A. C.freundii B. C.koseri C. Salmonella spp.
B. C.koseri
47
Sources of infection: water, milk, DAIRY PRODUCTS, shell fish, DRIED OR FROZEN EGGS, meat and meat products and houshold pets Cause enterics, systemic infection and enteric fever (typhoid fever) Motile LDC+ NLF H2S+ IMVIC -+-+ TSIA: K/A H2S+ A. C.freundii B. C.koseri C. Salmonella spp
C. Salmonella spp.
48
Associated with Typhoid fever IMVIC: -+-- TSIA: K/A GAS- H2S+ Black colonies with metallic sheen on BSA/ Bismuth sulfite Agar A. Salmonella serotype Typhi B. Salmonella serotype Enteriditis
A. Salmonella serotype Typhi
49
Associated with infections acquired from the ingestion of EGGS OR CHICKENS A. Salmonella serotype Typhi B. Salmonella serotype Enteriditis
B. Salmonella serotype Enteriditis
50
tube dilation aggl'n test:
Widal's test
51
In Widal's test, at least 2 serum specimens obtained at intervals of ____-____ days, are needed to prove a rise in antibidy titer A. 5-8 days B. 6-9 days C. 7-10 days D. 8-11 days
C. 7-10 days
52
High or rising titer of O>1:160 suggest _____ infection is present A. Past B. Active
B. Active
53
High titer of H>1:160 suggest _____ infection A. Past B. Active
A. Past
54
IMVIC:-+-- Nonmotile Lysine decarboxylase - Citrate - Malonate- H2S - Non- LF Gram Neg Rods that grow well on MacConkey agar A. Yersinia spp. B. Shigella spp. C. Pseudomonas spp.
B. Shigella spp.
55
Group A: Catalase - ONPG - MANNITOL - A. S. boydii B. S. dysentriae C. S. flexneri D. S. sonnei
B. S. dysentriae
56
Group B Catalase + ONPG - MANNITOL + A. S. boydii B. S. dysentriae C. S. flexneri D. S. sonnei
C. S. flexneri
57
Group C: Catalase + ONPG - MANNITOL + A. S. boydii B. S. dysentriae C. S. flexneri D. S. sonnei
A. S. boydii
58
Group D Catalase + ONPG +, LATE LF MANNITOL + A. S. boydii B. S. dysentriae C. S. flexneri D. S. sonnei
D. S. sonnei
59
Gram Negative Rods exhibit an unusual BIPOLAR STAINING ( weison stain) Catalase - Oxidase - Indole + Non-LF Facultative anaerobes capable of growth at temp. Ranging from 4°C to 43°C A. Yersinia spp. B. Shigella spp. C. Pseudomonas spp.
A. Yersinia spp. * REMEMBER! " BIPOLAR si BAY" B-urkholderia A-. actinomycetemcomitans Y- ersinia
60
Most commonly transmitted by the BITE of an infected rat flea (Xenopsylla cheopsis) resulting in BUBONIC PLAGUE; PNEUMONIC PLAGUE Bioterrorism, NOT included in ENTEROBACTERIACEAE termed as "BLACK DEATH" broth culture: exhibit a STALACTITE PATTERN Inclusion: BIPOLAR Stain Stain: wayson stain ( SAFETY PIN APPEARANCE) A. Y. pseudotuberculosis B. Y. enterocolitica C. Y. Pestis
C. Y. Pestis
61
Found in GI Tract of Swine, Rodents, Dogs Can cause acute mesenteric lymphadenitis, which may present as APPENDICITIS ENTEROCOLITIS: FEVER DIARRHEA AND ABDOMINAL PAIN (appendicitis like) Culture: CIN (BULLSEYE appearance) UREASE POSITIVE! MOTILE AT ROOM TEMP NON-MOTILE AT 37°C A. Y. pseudotuberculosis B. Y. enterocolitica C. Y. Pestis
B. Y. enterocolitica
62
Found in variety of wild and domesticated animals including Rodents, Birds and Rabbits Infection similar to Y.enterocolitica but less common A. Y. pseudotuberculosis B. Y. enterocolitica C. Y. Pestis
A. Y. pseudotuberculosis
63
Most common cause of death associated with BACTERIAL CONTAMINATION OF PACKED RBC A. Y. pseudotuberculosis B. Y. enterocolitica C. Y. Pestis
B. Y. enterocolitica *CIN AGAR / YERSINIA SELECTIVE AGAR
64
Bull's neck INFECTION: A. Y.enterocolitica B. Enterobacter C. Borrelia burgdorferi D. E.histolytica E. C.diphteriae
E. C.diphteriae
65
Bull's eye RASH (Erythema chronicum migrans): A. Y.enterocolitica B. Enterobacter C. Borrelia burgdorferi D. E.histolytica E. C.diphteriae
C. Borrelia burgdorferi
66
Bull's eye AMOEBA: A. Y.enterocolitica B. Enterobacter C. Borrelia burgdorferi D. E.histolytica E. C.diphteriae
D. E.histolytica
67
Bull's eye COLONIES on CIN: A. Y.enterocolitica B. Enterobacter C. Borrelia burgdorferi D. E.histolytica E. C.diphteriae
A. Y.enterocolitica *Also Aeromonas
68
FISH eye COLONIES on EMB: A. Y.enterocolitica B. Enterobacter C. Borrelia burgdorferi D. E.histolytica E. C.diphteriae
B. Enterobacter *Pink colonies w/ dark center
69
READ AND REMEMBER! " SPACEd" = H2S POSITIVE S-ALMONELLA P-ROTEUS A-RIZONAE C-ITROBACTER FREUNDII Ed-wardsiella tarda
70
TSIA: K/A GAS - H2S - " SPPY" S-higella P-rovidencia P-lesiomonas Y- ersinia TSIA: K/A GAS+ H2S- "MCKS" M-organella C-itrobacter K-oseri S-erratia TSIA: K/A GAS+ H2S+ "PECS" P.mirabilis E. tarda C. Freundii Salmonella TSIA : A/A GAS+ H2S - "EKE" E.coli K-lebsiella E-nterobacter TSIA: K/K GAS- H2S- "PA" P-seudomonas A-lcaligenes
71
ENTEROBACTERIACEAE MEMORIZE THIS! INDOLE + " May PEKPEC Po"? M-organella P-roteus vulgaris E.coli K.oxytoca P-rovidencia E. tarda C-itrobacter koseri P-lesiomonas VP+ "KEESH" K-lebsiella E-wingella E-nterobacter S-erratia H-afnia Citrate + "Pero Sa CHEEKS" P-rovidencia S-erratia C-itrobacter H-afnia E-wingella E-nterobacter K-lebsiella S-almonella enteritidis
72
MEMORIZE THIS! Rapid UREASE Producer: "PPM" P-roteus P-rovidencia rettgeri M-organella Other RAPID UREASE Producer: "TPUNCH" T. mentagrophytes (fungi) P-PM (grp.) U-reaplasma N-ocardia C.neoformans (fungi) H.pylori (peptic ulcer) SLOW UREASE Producer "CKEYS" C-itrobacter K-lebsiella E-nterobacter gergoviae Y. enterocolitica S-erratia

tagumpay Sa MARCH 2024! (19 decks)

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