Fatty Acid Metabolism

Question 1

The correct sequence of steps for shortening of a fatty acyl CoA by two carbons is:
A. Dehydration, hydration, oxidation, thiolytic cleavage.
B. Thiolytic cleavage, hydration, oxidation, acylation.
C. Oxidation, hydration, oxidation, thiolytic cleavage.
D. Oxidation, hydration, dehydration, thiolytic cleavage.
E. Hydration, oxidation, oxidation, thiolytic cleavage.

Answer 1

C. Oxidation, hydration, oxidation, thiolytic cleavage

Question 2

Beta-oxidation of an odd-chain fatty acid produces several two-carbon and one three-carbon molecules. The latter enters the TCA cycle in the form of which compound?
A. Citrate
B. Isocitrate
C. alpha-ketoglutarate
D. Succinyl CoA
E. Malate

Answer 2

D. Succinyl CoA

Question 3

Synthesis of fatty acid involves a series of reactions all of which occur on the fatty acid synthase complex. Which of the following most accurately represents the sequence of these reactions?
A. Condensation, reduction, dehydration, reduction.
B. Condensation, reduction, hydration, reduction.
C. Condensation, oxidation, dehydration, oxidation.
D. Condensation, oxidation, hydration, oxidation.

Answer 3

A. Condensation, reduction, dehydration, reduction.

Question 4

A pharmaceutical company is developing a new drug that inhibits fatty acid synthesis in obese (body mass index [BMI] > 30) patients. The drug is supposed to prevent the insulin-dependent activation of the rate limiting enzyme of fatty acid synthesis. To check the efficacy of drug candidates in cell cultures, the inhibition of which of the following enzymes would serve as a suitable marker?
A. Long-chain acyl CoA dehydrogenase (LCAD)
B. Protein phosphatase
C. Glycogen phosphorylase
D. Protein kinase B (PKB)
E. Protein kinase A (PKA)

Answer 4

B. Protein phosphatase