FILARIAE

Question 1

state filarial spp which are pathogenic

Answer 1

Wuchereria bancrofti
Brugia malayi
Loa loa
Onchocerca volvulus

Question 2

state filarial spp which are nonpathogenic

Answer 2

Mansonella ozzardi
Mansonella perstans

Question 3

What are the two known morphologic forms of filarial spp

Answer 3

Adult worms
Microfilariae (larvae)

Question 3

where are falarial spp mostly seen

Answer 3

within blood or tissues

Question 4

Filarial spp are under the phylum?

Answer 4

Nemathelminthes

Question 5

Filarial spp are under the class?

Answer 5

Nematoda

Question 6

creamy white and threadlike appearance

Answer 6

adult worms

Question 7

slender and size ranges under 150 um to 350 um in length

Answer 7

microfilariae

Question 8

state the two key characteristics helpful in speciating the microfilariae forms

Answer 8

distribution of nuclei
presence or absence of sheath

Question 9

True or False. The Life Cycle is same for all members of the filariae

Answer 9

True

Question 10

How many infective larvae/microfilariae is required to initiate human infection

Answer 10

1 to 4

Question 10

resulting adult worms reside in

Answer 10

lymphatics
subcutaneous tissue
intestinal body cavities

Question 11

where to live microfilariae take up residence

Answer 11

blood or dermis

Question 11

who lays live microfilariae

Answer 11

fertilized adult FEMALE worms

Question 11

is there periodicity in filarial spp

Answer 11

Yes

Question 12

a pheno menon whereby the parasites are present in the bloodstream during a specific time

Answer 12

periodicity

Question 13

parasite is present in the blood during the NIGHT

Answer 13

nocturnal

Question 14

parasite is present in the blood during the day

Answer 14

diurnal

Question 14

whole blood sample may also be collected via

Answer 14

knott technique
membrane filtration

Question 15

timing of the occurences is not clear-cut

Answer 15

Subperiodic

Question 15

primary method of diagnosis of filarial spp

Answer 15

microscopic examiniation of the microfilariae of either from:
giemsa stained blood
tissue scrapping of an infected nodule

Question 16

composition of knott technique

Answer 16

1 ml of blood mixed with 140 ml of 2% formalin

Question 17

state the process of the knott technique

Answer 17

1 ml of blood mixed with 140 ml of 2% formalin; centrifuged; supernatant is discarded; sediment is studied and spread like a thin blood film; fixed and stained and is then view under the microscope

Question 17

this makes use of syringe attached to a Swinney filter holder

Answer 17

membrane filtration

Question 18

membrane filtration composition

Answer 18

1 ml of fresh/anticoagulated blood (in syringe) & lyzed by adding 10ml distilled water > lyzed blood passes through the Swinney membrane filter > microfilariae is recovered

Question 19

true or false. membrane filter can be examined like a wet smear prep, fixed, dried, stained

Answer 19

true

Question 20

microfilariae in blood

Answer 20

microfilaremia

Question 21

concern regarding serologic tests

Answer 21

specificity (they are not specific)

Question 22

common name for Wuchereria bancrofti

Answer 22

Bancroft's Filaria

Question 23

common associated disease for Wuchereria bancrofti

Answer 23

Bancroft's Filariasis or Elephantiasis

Question 24

More sensitive method for W. bancroft microfilariae recovery

Answer 24

heparinized blood | blood filtered nucleopore filter filtered content are stained & examined

Question 24

peak hours for collecting the specimen infected w/ W. bancrofti

Answer 24

9pm to 4 am (nocturnal)

Question 24

serological tests used in W. bancrofti

Answer 24

polymerase chain reaction assays antigen & antibody detection

Question 25

this may occur in asymptomatic patients of W. bancrofti

Answer 25

eosinophilia

Question 26

this may occur in on the death of W. bancrofti adult worms

Answer 26

calcification or formation of abscesses

Question 27

bancroftian filariasis results to

Answer 27

formation of granulomatous lesions lymphangitis lymphadenopathy

Question 28

common name for Brugia malayi

Answer 28

Malayan Filaria

Question 29

Common associated disease for Brugia malayi

Answer 29

Malayan filariasis or Elephantiasis

Question 30

Common associated disease for Loa loa

Answer 30

Loiasis