FINAL 01 - Chromatography Flashcards
Is the ability to separate molecules using various partitioning characteristics of a molecule
Chromatography
Chromatography was first applied by __________ for the separation of plant pigments
Mikhail Semyonovich Tsvet
Greek word which means ‘color’
Chromatos
Greek word which means ‘to write’
Graphein
2 major components of chromatographic separation
Mobile phase, Stationary phase
Flows through the column; carries analyte
Mobile phase
Stays in place, does not move
Stationary phase
Instrument employed for a chromatography
Chromatograph
Phase that stays in place inside the column
Stationary phase
The solvent moving through the column; either a liquid in LC or gas in GC
Mobile phase
Fluid entering the column
Eluent
Fluid exiting the column
Eluate
Is the process of passing the mobile phase through the column
Elution
Graph showing detector response as a function of time
Chromatogram
Defines how much mobile phase passes per minute (mL/min)
Flow rate
Distance passed by mobile phase per 1 min in the column (cm/min)
Linear velocity
Red pigment in leaf
Anthocyanins
Orange pigment in leaf
Carotenoids
Yellow pigment in leaf
Xanthophyll
Green pigment in leaf
Chlorophyll
Rf value of beta-carotene
0.99
Rf value of chlorophyll A
0.30
Rf value of chlorophyll B
0.13
Rf value of violaxanthin
0.40
