final Flashcards
(25 cards)
what is differential labeling
a method used to differently label 2 molecules of interest in an experiment so that investigators are able to distinguish btwn 2 molecules in the results
what are 2 molecules that they were able to label in their experiment
dna and protein
how did they label they molecule that was passed on to the next generation of phage
sulfur
how did they label the molecules that were associated with phage ghosts after the experiment
P32
what is the impact of having a nucleus on the creation of mature mrna in prokaryotes
because prokaryotes do not have a nucleus they translational machinery is able to attach to the mrna as it is being synthesized. this there is no time to process the mrna
what is the impact of having a nucleus on the creation of mature mrna in eukaryotes
in eukaryotes the translational machinery is not present in the nucleus. thus there is time for the cell to process the mrna before it is transported from the nucleus to the cytoplasm and translated into protein.
central dogma
replication, storage, expression
what is the definition of biologically active dna in this context
dna that can be transcribed and translated and ultimately code for a viable protein.
dna polymerase III adds nucleotides
to the 3’ end of the rna primer
which of the following terms accurately describes the replication of dna in vivo ?
semidiscontinous
which of the following enzymes are known to be involved in the replication of dna in bacteria ?
dna polymerase I, DNA ligase, rna primase
which are characteristics of DNA sequence at the telomeres
- they consist of cytosine and adenine nucleotides
- the consist of repeated sequences
- one strand protrudes beyond the other, creating some single stranded dna at the end
the term peptidyltransferase relates to
peptide bond formation during protein synthesis
an intron is a section of
rna that is removed during rna processing
in eukaryotes trna are
transcribed in the nucleus but function in the cytoplasm
reverse transcriptase is an enzyme found in association with retroviral activity it has the property of
synthesis of dna from rna template
What would Avery, Macleod, and McCarty have concluded if their results had been that only RNAse tréatment of the heat-killed bacteria prevented transformation of genetic virulence?
the rna is the genetic material
which of the following statements best describes the mrna
mrna is the only type of rna that carries dna protein building instructions
dna consists of repeating units of nucleotides. which is not a component of a nucleotide
a ribose sugar
dna replication the lagging strand is
the strand which must be replicated In the direction away from the replication fork
the spliceosome is a large enzyme that
catalyzes removal of specific introns in the pre mrna
eukaryotic chromosomes contain two general domains that relate to the degree of condensation regions are
called heterochromatin and euchromatin
what enzyme is exploited to produce synthetic mrna
polynucleotide phosphorylase
the anti codon on the trna molecule
binds to the mrna in complementary fashion
