final exam Flashcards
(28 cards)
1
Q
What is the 5’ overhang
A
5’ protruding ends
hanging phosphate group
2
Q
What is the 3’ overhang
A
3’ protruding ends
hanging hydroxyl group OH
3
Q
what is blunt end endonuclease
A
4
Q
what is the characteristics of restriction endonuclease
A
5
Q
how is restriction enzyme used in molecular diagnostics
A
5
Q
how does star activity look on a gel
A
5
Q
How does restriction enzyme cut DNA
A
5
Q
What is the most used restriction enzyme?
A
type 2
5
Q
what is star activity
A
5
Q
Type 2 restriction enzyme
A
cleavage at specific recognition sites
ONLY unmethylated
5
Q
What are the two sticky ends of restriction enzyme in the lab
A
5’ overhang and 3’ overhang
5
Q
What are the three ways to cut
A
5’ overhang
3’ overhang
blunt end
5
Q
A
5
Q
A
6
Q
A
6
Q
A
6
Q
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6
Q
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6
Q
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6
Q
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6
Q
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6
Q
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6
Q
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6
Q
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