Final Exam Flashcards
(96 cards)
what are the main parts of a virus?
a nucleic acid (Rna or Dna) with protein coat surrounding it (capsids)
How are hosts important in viral replication
viruses can’t replicate on their own, they rely on hosts
what is a bacteriophage?
viruses that infect and replicate only in bacterial cells.
lytic cycle
ATTACHMENT: attachment of virus onto host cell provided the virus can chemically recognize and lock onto specific groups on the cell’s surface
PENETRATION: whole virus or genetic material from virus enters host cells cytoplasm
REPLICATION/SYNTHESIS: DNA or RNA directs the host cell to make many copies of viral components
ASSEMBLY: viral nucleic acids and viral proteins are assembled into new viruses
RELEASE: host cell bursts to release new virus particles
Lysogenic pathway
cycle is extended by latent period
1. Viral Dna is integrated into bacterial chromosome
2. Bacterium replicates all DNA including that of virus
3. After binary fission, each daughter cell has chromosome with viral DNA incorporated
4. Later, a molecular signal or other stimulus may reactivate the viral DNA. Then goes through lytic cycle
what type of virus is HIV? what does it cause? how does HIV compromise the immune system?
retrovirus, causes aids, compromises the immune system by targeting CD4+ cells/ T Helper cells. These are responsible for mounting a defense against invading bacteria or viruses.
New viruses are made each year to combat influenza virus. Explain
flu viruses mutate (undergo antigenic shifts) making it difficult to make effective vaccines. Different strains of subtypes vary in antigenic protein spikes
what are emerging viruses?
viruses that originate in one organism then pass to another and cause disease
how do viruses cause cancer?
viruses can trigger oncogenes(cancer cells) to become active. May also carry and integrate oncogenes into host cells genome.
What are prions? what kinds of diseases are caused by prions?
prions are misfolded proteins that is an infectious particle. Prion’s cause TSE’s which is scrapie in sheep
What are viroids? What kinds of diseases are caused by viroids?
infectious RNA with no protein coat; infect plants; use host protein to replicate
What are features of prokaryotic cells?
unicellularity, make up domains bacteria and archaea, small circular dna usually have plasmids, cell division by binary fission, no membrane bound organelles, singular flagellum
What types of organisms are in domain Archaea?
methanogens (produce methane gas) halophiles (salt loving organsims) thermoacidophiles (organisms thriving in hot, acidic environments)
distinguish between gram-positive and gram-negative bacteria
gram positive: thicker peptidoglycan wall, stains purple
gram negative: less peptidoglycan; don’t retain purple dye; red counter dye results in dark pink color, outer membrane contains lipopolysaccharides
what are 3 basic forms or shapes of prokaryotes?
rod-shaped, cocci, spiral
what are plasmids? how are they involved with conjugation?
small accessory DNA molecules that many bacteria possess. transferred through direct physical contact between bacteria (conjugation) helps bacteria share their antibiotic resistance genes with neighbors
what is transduction?
phages carry prokaryotic genes from one host cell to another. Transduction results from accidents that occur during phage replicative cycle. Method uses viruses
what is transformation?
genotype and possibly phenotype of prokaryotic cell are altered by uptake of foreign DNA from surroundings. bacteria pick up genetic material from environment
photoautotrophs
photosynthetic bacteria
example is cyanobacteria
chemolithoautotrophs
autotrophic microorganism that obtains energy by oxidizing inorganic compounds
photoheterotrophs
use light as source of energy but obtain carbon from organic molecules. Example are purple and green nonsulfur bacteria
chemoheterotrophs
obtain both carbon and energy from organic molecules. Example are majority of prokaryotes including decomposers and most pathogens
How are prokaryotes involved in cycling important elements such as carbon and nitrogen?
Fixation returns elements from inorganic form to organic forms that heterotrophs use. Carbon fixation involves incorporating the carbon CO2 into complex organic compounds. Nitrogen fixation involves certain bacteria converting N2 gas to ammonia NH3 and nitrate NO3
how are bacteria used in genetic engineering
production of pharmaceutical agents such as insulin. used in production of enzymes, vitamins, and antibiotics