Final Exam Jeopardy

Question 1

Predominant normal flora of the adult large intestine

Answer 1

Anaerobic organisms

Question 2

Pathogenic organisms that should be ruled out in a stool culture in the USA

Answer 2

Campylobacter, Salmonella, Shigella, and E.coli O157:H7

Question 3

Media that should be set up on a stool culture

Answer 3

BAP, MAC, HE/XLD, CAMPY, MAC SORB, GN broth

Question 4

Transport media and temperature for stool specimen

Answer 4

Cary Blair at refrigerator temp.

Question 5

Salmonella colony morphology on MAC and HE

Answer 5

MAC = NLF
HE = H2S positive

Question 6

Media to set on Vag culture

Answer 6

BAP, CHOC, MAC, MTM

Question 7

GNDC
OX +
Glu +
MAL -
SUC -
LAC -

Answer 7

N. gonorrhoeae

Question 8

Incubation conditions for wound cultures

Answer 8

35 C, CO2, Anaerobic plates incubated anaerobicallly

Question 9

Organisms most frequently associated with impetigo

Answer 9

S. aureus
Group A Streptococcus

Question 10

Media to set for an abscess aspirate specimen

Answer 10

BAP, CHOC, MAC, CNA, THIO, ANA

Question 11

How to handle Sputum specimen with gram stain with >10 sep/lpf and no WBC’s

Answer 11

reject the specimen

Question 12

organism in sputum culture
alpha hemolytic, GPC, cat -, Optochin Sensitive

Answer 12

S. pneumoniae

Question 13

Media to set on sputum culture and incubation condition

Answer 13

BAP, CHOC, MAC
35C in CO2

Question 14

Causative agent of epiglottitis

Answer 14

H. influenzae

Question 15

Common conditions associated with S. pyogenes

Answer 15

pharyngitis, rheumatic fever, impetigo

Question 16

Collection container and transport requirements for urine culture

Answer 16

sterile container transported at 4 C or in a boric acid tube at room temp.

Question 17

Loop used to set a routine urine culture and most common media

Answer 17

0.001 ML loop
BAP, MAC

Question 18

Storage condition for CSF specimen that can’t be processed immediately

Answer 18

Room temp or 35C

Question 19

CSF specimen isolate, Small gnb, No growth on BAP/MAC, grows on chocolate, Ox + (slow)
Next step for identification and expected organism

Answer 19

Set X, V, XV, rabbit blood
Expect H. influenzae

Question 20

Common urinary pathogens

Answer 20

Entoerococcus, E. coli, S. saprophyticus, other enteric gnb, S. aureus,

Question 21

Toxin producing anaerobe that can cause diarrhea after patient’s antibiotic treatment

Answer 21

C. difficile

Question 22

Best collection time for detection of Mycobacterium in sputum

Answer 22

first morning specimen

Question 23

Primary atypical pneumonia is caused by

Answer 23

Mycoplasma pnumoniae

Question 24

Appearance of Mycoplama hominis on specialized agar

Answer 24

Fried eggs

Question 25

Infectious form of Chlamydia trachomatis

Answer 25

Elementary body