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Clinical Microbiology WSSU > Final Exam Study > Flashcards

Final Exam Study Flashcards

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1
Q

Lack of motility is characteristic of:

  • Klebsiella oxytoca
  • Proteus mirabilis
  • Escherichia coli
  • Enterobacter cloacae
A

Klebsiella oxytoca

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2
Q

You are the technologist working in the microbiology department today. You perform a Gram stain from a sample submitted from a 65 year old man with urethral discharge.

Gram Stain: Gram negative diplococci

Based off the gram stain, what is the next step to help identify the organism?

A

Inoculate onto a thayer martin media and incubate in 35-37 C in an increased CO2 environment

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3
Q

Some strains of Serratia marcescens produce a red colored pigment. Pigment production is enhanced by:

A

Incubation at 22 C

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4
Q

The enterotoxin produced by certain strains of hemolytic, coagulase positive Staphylococcus aureus

  • Are not involved in causing harm
  • Can cause bacterial food poisoning
  • Are only of one type of toxin
  • Can be destroyed by boiling for 5 mins
A

Can cause bacterial food poisoning

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5
Q

A urethral swab obtained from a man with a urethral exudate was plated directly on chocolate agar and modified Thayer-Martin agar, and a Gram stain was made. The Gram stain showed Gram negative diplococci. The culture plates were incubated at 35°C, but had no growth at 48 hours. The most likely failure for organism growth is that the:

A

Organism requires CO2 for growth

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6
Q

Infection of the urinary tract is most frequently caused by:

A

Escherichia coli

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7
Q

The primary isolation of Neisseria gonorrhoeae requires:

A

Carbon dioxide

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8
Q

It is important to differentiate between Enterococcus and Group D streptococci because:

A

Enterococci often show more antibiotic resistance

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9
Q

The results of biochemical testing on the above-pictured organism (LF mucoid colonies) are:
- Urease positive
- Citrate positive
- This organism was non-motile

All of the above results are most likely representative of:

A

Klebsiella spp.

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10
Q

After 24 hrs a blood culture from a newborn grows a catalase-negative, Gram positive cocci. The bacterial colonies are small, translucent, and beta-hemolytic on a blood agar plate. Biochemical test results of a pure culture are:

  • Bacitracin resistant
  • CAMP reaction positive
  • Bile esculin not hydrolyzed
  • 6.5% NaCl broth: no growth

What is the next step?

A

Perform Streptococcus group testing

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11
Q

A common cause of ear infections in children is:

A

Streptococcus pneumoniae

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12
Q

Untreated streptococcal pharyngitis can lead to all of the following except:

  • Common cold
  • Rheumatic fever
  • Scarlet fever
  • Acute glomerulonephritis
A

Common cold

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13
Q

This is a growth (pinpoint colonies with large zone of beta hemolysis) from a blood agar plate from a throat culture of a 6 year old patient with the following biochemical results:

  • Catalase negative
  • Susceptible to bacitracin
  • PYR positive
  • Bile esculin negative
  • 5% NaCl negative

Which of the following statements can correlate with these results?

  • this organism can be the cause of scarlet fever
  • patient presents with fever, red raw throat, and headache
  • this organism can cause damage to the glomerulus due to immune complex deposits in the glomerulus
  • All of the statements can correlate with these results
A

All of these statements can correlate with these results

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14
Q

A positive Voges-Prokauer reaction is characteristic of:

A

Enterobacter aerogenes

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15
Q

An isolate of an unknown beta-hemolytic Streptococcus is streaked perpendicular to a streak of beta lysin producing Staphylococcus aureus. After incubation, a zone of arrowhead hemolysis is noted at the interface of the two streaks. What is the name of the test and the presumptive identification of the unknown Streptococcus?

A

a CAMP test, Streptococcus agalactiae

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16
Q

Members of the order Enterobactrales share which one of the following characteristics?

  • Produce beta hemolysis
  • Are oxidase positive
  • Ferment lactose
  • Reduce nitrates to nitrites
A

Reduce nitrates to nitrites

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17
Q

Clinical resistance to penicillin correlates most frequently with beta-lactamase production in:

A

Neisseria gonorrhoeae

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18
Q

A urine Gram stain show Gram positive cocci in clusters. The organism tested as catalase positive. To help determine the species the technologist should perform a coagulate test and a/an

  • oxidase
  • Polymyxin B susceptibility
  • Novobiocin susceptibility
  • Beta-lactamase
A

Novobiocin susceptibility

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19
Q

The K antigen of the family Enterobacteriaceae is:

A

Heat labile

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20
Q

Children who have infections with beta-hemolytic streptococci can develop:

A

Acute glomerulonephritis

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21
Q

How many hours after eating contaminated food do initial symptoms of staphylococcal food poisoning typically occur?

A

2-6

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22
Q

An organism previously thought to be nonpathogenic, Moraxella catarrhalis, is now known to be associated with an opportunistic respiratory infection and nosocomial transmission. Characteristic identification criteria include:

  • Carbohydrates negative
  • Beta lactamase negative
  • Gram negative bacillus
  • Oxidase negative
A

Carbohydrates negative

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23
Q

Conglutination is associated with:

A

Neisseria gonorrhoeae

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24
Q

A reliable test for distinguishing Staphylococcus aureus from other staphylococci is:

A

Coagulase

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25
A gram positive coccus isolated from a blood culture has the following characteristics: - optochin susceptibility: negative - Bacitracin (0.04U) susceptibility: negative - Bile esculin hydrolysis: negative - Hippurate hydrolysis: positive - Catalase: negative The organism is most likely: - Strep. pneumoniae - Strep. agalactiae - Strep. pyogenes - Staph. aureus
Strep. agalactiae
26
The optochin disk is used for the identification of:
Strep. pneumoniae
27
A urine culture from a 23 year old female grew a catalase-positive Gram positive coccus, which would most likely be:
Staph saprophyticus
28
Shigella species characteristically are:
non motile
29
Hemophilus influenzae becomes resistant to ampicillin when the organism produces a(n):
beta lactamase enzyme
30
A mother brings her lethargic, feverish baby into the emergency department. The baby is diagnosed with meningitis and the doctor does a spinal tap. The cerebrospinal fluid gram stain shows many tiny gram negative rods. What is the most probable identification of the organism?
Haemophilus influenzae serotype B
31
If present, a characteristic that is helpful in separating Pseudomonas aeruginosa from other members of the Pseudomonas family is:
Production of the pigment, pyocyanin
32
This organism can be found in raw or improperly cooked oysters. It is usually self-limiting. It produces green colonies on TCBS agar, grows well in high NaCl content and is negative for lactose fermentation.
Vibrio parahaemolyticus
33
A college student attended a beach party where raw oysters and other shellfish were consumed. The next day, he had symptoms of septicemia. The blood cultures grew gram negative bacilli with the following characteristics: - Oxidase pos - MacConkey agar: pink colonies - O/129 (150 micrograms): susceptible
Vibrio vulnificus
34
How is Helicobacter pylori presumptively identified?
A rapid urease test done on a gastric biopsy
35
Campylobacter spp. Gram stain is described as tiny Gram negative rods, curved, with S-shaped or seagull wing shapes. What important step is needed when performing the Gram stain for this organism?
Extend the safranin time to 3 mins
36
What organism is the likely cause of infection after a cat bite?
Pasteurella spp.
37
Thiosulfate citrate bile salt sucrose agar is used to isolate and differentiate:
Vibrio
38
Characteristics of the HACEK group of bacteria include:
requirement of 5-10% carbon dioxide for growth
39
What biochemical test will differentiate nonfermenters from Enterobacteriaceae (except Plesiomonas)
Oxidase
40
A technician is reading the plates from a sputum culture. On the sheep blood agar, she sees flat spreading colonies with a metallic sheen. On MacConkey, she sees medium clear colonies that have a fruity or grape-like odor. What is the most likely organism?
Pseudomonas aeruginosa
41
Which species of Heamophilus requires both X and V factors?
Haemophilus influenzae
42
What is the specimen of choice for Bordetella species?
Nasopharyngeal swabs
43
This organism is associated with human bite wounds. It is a Gram negative bacilli and show characteristic pitting on blood agar. It has a bleach-like odor.
Eikenella corrodens
44
The _______ vaccination is to prevent ________ which is caused by Bordetella pertussis.
DPT/Whooping cough
45
What organism is associated with pneumonia in patients with cystic fibrosis or chronic granulomatous disease (CGD)?
Burkholderia cepacia
46
What is the name of the organism that is strongly associated with gastric, peptic, and duodenal ulcers as well as GI carcinoma?
Helicobacter pylori
47
Which of the following does not produce pyoverdin pigment?
Burkholderia cepacia
48
What organisms is the most common cause of bacterial gastroenteritis worldwide and is unique with its "darting" motility in the hanging drop test?
Campylobacter jejuni
49
Which of the following is a media that can be used to isolate Legionella?
BCYE
50
What organism is the highly contagious causative agent of chanroid? The gram stain resembles a school of fish.
Hemophilus ducreyi
51
the single species of Plesiomonas, P. shigelloides is similar to Vibrio spp. in that they're both O/129... sensitive or resistant?
Sensitive
52
What organism causes melioidosis?
Burkholderia pseudomallei
53
A small, gram negative bacillus is isolated from an eye culture. It grows only on chocolate agar and is oxidase variable. The most likely organism is:
Hemophilus influenzae
54
CAMPY blood agar plates contains:
Brucella agar base, 10% sheep RBCs, vancomycin, trimethoprim, polymyxin B, amphotericin B and cephalothin
55
On Bordet-Gengou medium, Bordetella pertussis appears as:
Smooth and shiny, resembling mercury droplets
56
Legionnaires' disease is spread through:
Inhalation of aerosols from contaminated water
57
An organism producing a yellow color in the open tube and a blue-green color in the closed tube of an OF glucose determination is said to:
Oxidase glucose
58
A microbiology technologist is checking the mycobacteria cultures. She notices one tube of Lowwnstein-Jensen (LJ) that is buff color, rough, and seems arranged in a cord. It has taken these organisms 4 weeks to grow. What is the most probable organism?
Mycobacterium tuberculosisi
59
Several people from a picnic came down with a gastrointestinal disturbance, and public health officials suspected a case of food poisoning. All food from the picnic, including the hot dogs were cultured. After 24 hours, there were round, small, smooth, translucent colonies surrounded by a narrow zone of beta-hemolysis on sheep blood agar. A Gram stain showed Gram-positive coccobacillus. Motility medium was inoculated and an umbrella pattern of motility was observed at room temperature. What organism was isolated as the cause of the illness?
Listeria monocytogenes
60
What is the name of the bacteria that plays an important role in the health of the female vaginal tract, protecting it against pathogens?
Lactobacillus spp.
61
This Mycobacterium spp. is primarily found in cattle, dogs, and cats.
Mycobacterium bovis
62
What bacteria's morphology is described as large, square-ended, Gram positive, Gram variable rods, that sometimes create bamboo appearance when in chains?
Bacillus anthracis
63
In a suspected case of Hansen disease (leprosy), a presumptive diagnosis is established by:
detection of a weakly acid fast bacilli in infected tissue
64
What Bacillus species is a relatively common cause of food poisoning and opportunistic infection is susceptible hosts?
Bacillus cereus
65
A strict anaerobe that produces terminal spores is:
Clostridium tetani
66
What catalase-positive Gram positive spore-forming bacterium produces colonies that are non-hemolytic, large, gray, and flat with an irregular margin on sheep blood agar (SBA)?
Bacillus anthracis
67
What disease does Borrelia cause?
Lyme disease and relapsing fever
68
How is congenital syphilis transmitted from the mother to her unborn child?
by crossing the placenta
69
What two organisms are associated with urogenital tract infections and have also been isolated from asymptomatic individuals?
Mycoplasma hominis and Ureaplasma urealyticum
70
What organism causes endemic typhus?
Rickettsia typhi
71
A man is taking a shower and notices this lesion on his penis that is not tender, but firm with a clean surface and raised edges. He is quite dismayed so he goes to his physician. The physician does a rapid plasmin reagin (RPR) and darkfield microscopy. Both tests are positive. What is the physician's probable diagnosis?
Syphilis
72
What structure is responsible for motility in spirochetes?
Periplasmic flagella
73
What organism causes Rocky Mountain Spotted Fever?
Rickettsia rickettsii
74
Nontreponemal RPR testing for syphilis infections detect:
Wasseman antibodies (reagin)
75
What testing method is the method of choice for detecting antibodies to Chlamydia pneumoniae?
Serologic tests that show a 4 fold rise in titer
76
A young man goes to his doctor complaining of a discharge from his penis. The doctor collects a slide for Gram stain and a swab for culture and sends them to the lab. The Gram stain is prepared and read: >25 wbc/hpf are seen and no bacteria are seen. The culture results 48 hours later and shows no growth. What is the most probable diagnosis and the organism responsible?
Nongonococcal urethritis, Chlamydia trachomatis
77
Mycoplasma spp. are a different type of bacteria because they do not have a:
Cell wall

Clinical Microbiology WSSU (12 decks)

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