final Microbiology Flashcards
The minimal temperature, pressure and time to sterilize an object in a steam autoclave is:
100 degrees Celsius, 10 psi, for 15 minutes
100 degrees Celsius, 10 psi, for 30 minutes
121 degrees Celsius, 15 psi, for 15 minutes
170 degrees Celsius, 15 psi, for 1 hour
121 degrees Celsius, 15 psi, for 15 minutes
Acid-fast stained organisms appear what color?
orange
red
blue
green
red
Match each method of microbial control with the category it falls under physical,
Autoclaving
Irradiation
Freezing
Pasteurization
Match each method of microbial control with the category it falls under mechanical,
Filtration
Match each method of microbial control with the category it falls under chemical agent).
Ethylene oxide
Alcohols
Chlorhexidine
What color are non-acid-fast bacteria?
Pink
Red
Green
Blue
Blue
10 / 10 points
Place the steps of an acid-fast stain in order.
1.Cover smear with a piece of paper towel.
2.Saturate the paper with carbolfuchsin
3. Heat the slide for 5 minutes over a boiling water bath.
4.Remove the paper towel.
5 Rinse the slide with water for the 1st time
6.Decolorize the slide with acid alcohol.
7.Rinse the slide with water for the 2nd time
8.Counterstain the slide with methylene blue for 2 minutes.
9 Rinse the slide with water for the 3rd time
10.Blot the slide dry with bibulous paper
Decolorized non-acid-fast cells take up the carbolfuchsin stain.
True False
True
Which type of bacteria requires an acid-fast stain for them to be detected?
Clostridium perfringens
Streptococcus pneumoniae
Bacillus cereus
Mycobacterium tuberculosis
Mycobacterium tuberculosis
Increased temperatures increase the activity of a disinfectant.
True False
True
Kills all microorganisms on an object.
Sterilization
Reduces the numbers of or inhibits the growth of microorganisms.
Disinfection
Works on inanimate objects, is not safe for living tissues.
Disinfectant
Works on external body tissue, but cannot be taken internally.
Antiseptic
Steam under pressure.
Autoclave
