FINALS LAB 1: MICROSCOPE

Question 1

The simplest type of compound microscope

Answer 1

BRIGHTFIELD COMPOUND MICROSCOPE

Question 2

refers to the fact that magnified objects appear as dark objects against a bright background.

Answer 2

BRIGHTFIELD

Question 3

means that a specimen positioned properly on the stage of a microscope and illuminated by a light source will be magnified by a two-lens system

Answer 3

COMPOUND

Question 4

THE PATH OF LIGHT

Answer 4

1. ILLUMINATOR
2. CONDENSER
3. OBJECTIVE LENSES
4. OCULAR LENS

Question 5

THE PATH OF LIGHT
- also known as the LIGHT SOURCE

Answer 5

ILLUMINATOR

Question 6

THE PATH OF LIGHT
- has lenses that DIRECT THE LIGHT RAYS through the specimen

Answer 6

CONDENSER

Question 7

THE PATH OF LIGHT
- light rays then pass into the objective lenses, the LENSES CLOSEST TO THE SPECIMEN

Answer 7

OBJECTIVE LENSES

Question 8

THE PATH OF LIGHT
- then the image of the specimen is MAGNIFIED AGAIN BY THE EYEPIECE

Answer 8

OCULAR LENS

Question 9

oil used in oil immersion

Answer 9

CEDAR WOOD OIL

Question 10

CEDAR WOOD OIL REFRACTIVE INDEX

Answer 10

1.516

Question 11

The use of immersion oil improves the RESOLVING POWER OF THE LENSES

T OR F?

Answer 11

TRUE

Question 12

if oil is not used with an oil immersion objective lens, the image becomes ________, WITH POOR RESOLUTION

Answer 12

FUZZY

Question 13

PARTS OF THE MICROSCOPE
- houses the optical parts in the upper part of the microscope (STRUCTURAL COMPONENTS)

Answer 13

HEAD/BODY

Question 14

PARTS OF THE MICROSCOPE
- connects to the basse and supports the microscope head
- also used to carry the microscope (STRUCTURAL COMPONENTS)

Answer 14

ARM

Question 15

PARTS OF THE MICROSCOPE
- supports the microscope and houses the illuminator (STRUCTURAL COMPONENTS)

Answer 15

BASE

Question 16

PARTS OF THE MICROSCOPE
- remagnifies the image formed by the objective lens;
- STANDARD MAGNIFYING POWER: 10x (OPTICAL COMPONENTS)

Answer 16

EYEPIECE/OCULAR LENS

Question 17

PARTS OF THE MICROSCOPE
- holds the eyepiece in place above the objective (OPTICAL COMPONENTS)

Answer 17

EYEPIECE TUBE

Question 18

PARTS OF THE MICROSCOPE
- primary lenses that magnify the specimen (OPTICAL COMPONENTS)

Answer 18

OBJECTIVE LENSES

Question 19

PARTS OF THE MICROSCOPE
- a rotating turret that houses the objectives (OPTICAL COMPONENTS)

Answer 19

NOSEPIECE

Question 20

PARTS OF THE MICROSCOPE
- A BIGGER WHEEL used to adjust the LPO in focusing
- used for INITIAL FOCUSING

Answer 20

COARSE ADJUSTMENT

Question 21

PARTS OF THE MICROSCOPE
- a SMALLER WHEEL for FINAL FOCUSING of the specimen using HPO and OIO;
- used also to make the specimen MORE VIVID

Answer 21

FINE ADJUSTMENT KNOB

Question 22

PARTS OF THE MICROSCOPE
- where the specimen/slide is placed for focusing

Answer 22

STAGE

Question 23

PARTS OF THE MICROSCOPE
- used to hold the slide in place

Answer 23

STAGE CLIPS

Question 24

PARTS OF THE MICROSCOPE
- light source of the microscope

Answer 24

ILLUMINATOR

Question 25

PARTS OF THE MICROSCOPE - used to COLLECT and FOCUS the light from the illuminator on the specimen

Answer 25

CONDENSER

Question 26

PARTS OF THE MICROSCOPE - CONTROLS the amount of light reaching the specimen

Answer 26

IRIS DIAPHRAGM

Question 27

PARTS OF THE MICROSCOPE - moves the condenser up or down to control the lighting focus on the specimen

Answer 27

CONDENSER FOCUS KNOB

Question 28

OBJECTIVE LENSES: MAGNIFICATION: 4X BAND COLOR: RED

Answer 28

SCANNER

Question 29

OBJECTIVE LENSES: MAGNIFICATION: 10X BAND COLOR: YELLOW - usually forms the general outline or wide portion of the object

Answer 29

LOW POWER OBJECTIVE (LPO)

Question 30

OBJECTIVE LENSES: MAGNIFICATION: 40X BAND COLOR: BLUE - longer than the LPO and it forms a bigger image if the object is in focus - in most cases, it is used to ENLARGE specimens that are too small under LPO

Answer 30

HIGH POWER OBJECTIVE (HPO)

Question 31

OBJECTIVE LENSES: MAGNIFICATION: 100X BAND COLOR: WHITE - HIGHEST DEGREE OF MAGNIFICATION - used to examine STAINED SMEAR preparations of microorganisms using immersion oil as their medium.

Answer 31

OIL IMMERSION OBJECTIVE (OIO)

Question 32

CALCULATING THE MAGNIFICATION

Answer 32

TOTAL MAGNIFICATION = magnifying power of the eyepiece (10x) x magnifying power of the objective used