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Flashcards in FINDING DISEASE GENES Deck (9)
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1
Q

Probes do not have to be __ complementary.

A

100%

2
Q

What is a cDNA probe?

A

complementary only to exons

3
Q

How is DNA prepared for gels?

A

1) DNA is first acquired
2) then cut using restriction enzymes
3) loaded onto agarose gel
4) voltage applied
5) stained

4
Q

SDS- PAGE gel

A
  • nuclease digestion
  • keep cold to avoid degradation
  • charge affects mobility
  • antibody probe used
5
Q

SOUTHERN BLOTTING used for __(a)__ analysis. This is digested prior using restriction enzymes and fractionated onto gel. The absorbent ___(b)___ performs the ‘blotting’. Membrane bathed in labelled __(c)___.

A

a) DNA
b) membrane
c) probe

6
Q

NORTHERN BLOTTING used for __(a)___ analysis. Key point is that no __(b)___ is necessary.

A

a) RNA

b) digestion

7
Q

WESTERN BLOTTING involves transferring electrophoretically fractionated __(a)__ from a gel and visualising specific __(a)__ using __(b)___ probes. Also no __(c)___ required.

A

a) proteins
b) antibody
c) digestion

8
Q

Uses of RFLP mapping?

A
  1. can aim to map chromosomes
  2. distinguish disease gene loci in diagnostics
  3. measure genetic divergence
9
Q

Digested genomic DNA will usually appear as a continuous what?

A

smear