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Histopathology > FIXATION > Flashcards

FIXATION Flashcards

(58 cards)

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1
Q

Action of fixative where the fixative becomes part of the tissue

A

Additive

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2
Q

Action of additive fixative

A

Formation of crosslinks or complexes

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3
Q

Fixative not incorporated into the tissue

A

Non-additive

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4
Q

Action of non additive tissue fixative

A

Stabilizies tissue by removing water bound water

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5
Q

pH in fixation

A
  1. 0-8.0
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6
Q

pH in fixation

A
  1. 0-8.0
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7
Q

Temperature in regular fixation

A

Room temp (22-25)

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8
Q

Temperature of autotech fixation

A

40 C

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9
Q

Temp of EM and histochem fixation

A

0-4 C

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10
Q

Temperature in rapid fixation

A

60 C

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11
Q

Fixation temp in specimens with TB

A

100 c

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12
Q

Thickness of tissues in EM

A

1-2 mm

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13
Q

Thickness of tissue in LM

A

2cm

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14
Q

Tissue thickness should not be bigger than ____

A

4mm or 0.4cm

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15
Q

Brain tissue fixation prep suspended in ______

A

Circle of willis = 10 % buffered formalin 2-3weeks

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16
Q

Solution concentration in fixation

A

Hypertonic ( 400-450osm)

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17
Q

Ideal concentrating reagent in immunoelectrochemistry

A

0.25 glutaraldehyde

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18
Q

Time of fixation to prevent decomposition

A

Less than 1 hr

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19
Q

Rate of penetration of formalin

A

1mm/hr

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20
Q

Volume in fixation

A

20 times of tissue

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21
Q

Volume of fixative for expensive fixatives

A

5-10x of tissue

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22
Q

Volume for museum fixation

A

> 50x

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23
Q

Mortuary refrigerator temp

A

4c

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24
Q

Organ grossing is done after fixation

25
Organ Injected and cut after fixatiom
Eyes
26
Fixative that permit the general microscopic study of tissue structures without altering the structural pattern
Microanatomical
27
Preserves specific part or element of cell
Cytological
28
Needs glacial acetic acid
Nuclear
29
Effect of glacial acetic acid to cytoplasm
Swelling
30
Produced from oxidation of methanol
Formaldehyde
31
Pure stock of formalin sol
37-40%
32
Commonly used formalin sol
10%
33
Fixation time of formalin
12-24 hrs
34
Added to 10% formaline as buffer
PO4
35
Result of prolonged storaged of 10 formaline
Paraformaldehyde (white precipitate)
36
Black or brown precipitates im formaline solution in blood containing tissues
Acid formaldehyde hematein
37
Ideal for silver impregnation techniques
10% formaline
38
Best fixative for iron containing pigments
10% neutral buffered formaline
39
Recommended for routine histopath
10% neutral buffered formaline
40
Excellent for stains like reticulin mtds
Formol corrosive (formal sublimate)
41
Good for glycogen and microinceneration techniques
Gendre's
42
Fixes sputum
Gendre's
43
2 formaljne linked by 3 carbon chains
Glutaraldehyde
44
Recommended for enzyme histochem and EM
Glutaraldehyde
45
Polymer of formalin in white powder form
Paraformaldehyde
46
Used for ultra thin plastic embedding
Paraformaldehyde
47
Most common metallic fixative
Mercuric
48
Mercuric fixative that does not produce black granular deposits
Heidenhain susa
49
Removal for black deposits in mercuric chloride fixation
Dezenkerization
50
Routine fixative of choice for preservatiln of cell detail in tissue photography
Mercuric chloride
51
Mercuric chloride that contains glacial acetic acid
Zenker's
52
Mercuric chloride fixative Recommended for trichrome stain
Zenker's
53
Mercuric stain that produces brown pigments
Zenker formol
54
Excellent microanatomical fixative of pituitary gland, BM and blood containing organs
Zenker formol
55
Other name of zenker formol
Helly's
56
Mercuric stain recommended for skin tumor
Heidenhain susa
57
Mercuric fixatice with anhydrous Na acetate
B5 fixative
58
Mercuric stain for bone marrow
B5

Histopathology (2 decks)

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