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Histopathology (Lecture) Pathology & Fixative > Fixative > Flashcards

Fixative Flashcards

1
Q

Recommended for MAILING SPX and for COLORED TISSUE PHOTOGRAPHY

A

FORMALDEHYDE/FORMALIN

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2
Q

Removal of Formalin Pigments:
70% ethanol & 28% ammonia water

A

Kardasewitsch method

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3
Q

Removal of Formalin Pigments:
H2O2 + 28% ammonia water + Acetone

A

Lillie’s method

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4
Q

Removal of Formalin Pigments:
Saturated alcoholic picric acid

A

Picric acid method

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5
Q

Remedy for precipitation of white paraformaldehyde

A

10% Methanol

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6
Q

Distilled water w/ Sodium chloride (Histochemical fixative)

A

10% formol saline

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7
Q

Used for general post mortem tissues and CNS tissues

A

10% formol saline

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8
Q

10% formalin + phosphate buffer

A

10% neutral buffered formalin

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9
Q

Recommended for tissues w/ iron pigments and elastic fibers

A

10% neutral buffered formalin

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10
Q

Also known as Formol sublimate. Contains formaldehyde + mercuric chloride

A

Formal corrosive

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11
Q

Recommended for lipids, neutral fats, and phospholipids

A

Formal corrosive

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12
Q

Also known as Gender’s solution. 95% ETOH + Picric acid + Glacial acetic acid

A

Alcoholic formalin

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13
Q

For enzyme histochemistry and electron microscopy

A

Glutaraldehyde

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13
Q

Recommended for Sputum specimens and microincineration techniques

A

Alcoholic formalin

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14
Q

Glutaraldehyde solution % for small fragments and % for larger tissues less than 4mm thick

A

2.5% for small fragments; 4% for larger tissues

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15
Q

Glutaraldehyde solutions for electron microscopy

A

Karnovsky’s paraformaldehyde-glutaraldehyde solution and Acrolein

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16
Q

Supplied as 40% aqueous solution. Smallest aldehyde fixative

A

Glyoxal

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17
Q

Glyoxal fixative duration for Surgical specimens and Small biopsy specimens

A

4-6 hours; 45 mins

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18
Q

Most common metallic fixative

A

Mercuric chloride

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19
Q

Mercuric chloride + Glacial acetic acid.

Recommended for liver, spleen, connective tissue fibers, and nuclei.

A

Zenker’s fluid

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20
Q

Recommended for pituitary gland, bone marrow, blood containing organs

A

Zenker’s formol/ Helly’s fluid

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20
Q

Potassium dichromate + 40% formaldehyde

Also known as Helly’s fluid

A

Zenker’s formol/ Helly’s fluid

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21
Q

TCA + Glacial acetic acid + formalin

Recommended for tumor skin biopsies

A

Heidenhain’s susa

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22
Q

Anhydrous sodium acetate

For preserving bone marrow

22
Chromate fixative: preserves carbohydrates
(1-2%) Chromic acid
22
Mercuric acid fixative may form black mercury deposits, remedy?
wash with Alcoholic iodine
23
For early degenerative processes and tissue necrosis. For Rickettsia and other bacteria
Orth's fluid
23
Chromate fixative: preserves lipids and mitochondria
3% Potassium dichromate
23
Recommended for chromatin, mitochondia, mitotic figires, golgi bodies, rbc containing colloid tissues
Regaud's / Moller's fluid
23
Used for acid mucopolysaccharide and tissue mucin
Lead fixative
24
Acts as a Fixative, Stain, Decalcifying agent. Excellent in Glycogen demonstration
Picric acid fixatives
25
This fixative stains the tissue YELLOW as a major drawback
Picric acid fixatives
26
Recommended for embryos, pituitary biopsy, endometrial biopsy, endometrial curettings.
Bouin's solution
27
Excellent for Glycogen
Brasil's alcoholic picformol
28
For GIT biopsies and endocrine tissues
Hollande's solution
29
A compound fixative, recommended for nucleoproteins
Glacial acetic acid
30
Glacial acetic acid solidifies at ___ and contraindicated for cytoplasmic fixation
17 C
31
Acts as a Fixative and Decalcifying agent. A compound fixative.
Trichloroacetic acid (TCA)
31
Fixative is used at ice cold temperatures -5 to 4C
Acetone
32
Recommended for Enzymes and Brain tissues to diagnose Rabies
Acetone
33
Can Fix and Dehydrate at the same time
Acetone and Alcohol
34
Rapidly denatures and precipitates proteins
Alcohol
35
Ideal for small tissue fragments. Causes Glycogen polarization
Alcohol fixatives
36
Alcohol fixative: for wet and dry smears, blood smears, and bone marrow tissues
100% Methyl alcohol
37
Recommended for touch preparations
95% Isopropyl
38
Recommended for blood films, tissue films, and smears
70-100% Ethyl alcohol
39
Alcohol + Glacial acetic acid + chloroform
Carnoy's
40
Most rapid fixative. For chromosomes and lymph glands. w/ acetone to fix brain tissues to diagnose rabies
Carnoy's
41
Classified as both nuclear (nuclear proteins) and histochemical (mucopolysaccharide) fixative
Newcomer's
42
Recommended for CT mucins and umbilical cord
Rossman's solution
43
This fixative is Expensive, slow-acting, used in EM.
Osmium tetroxide
44
Processes Neurological tissues. For myelin and peripheral nerves
Osmium tetroxide
45
Most common chrome osmium acetic acid fixative. For nuclear structures
Flemming's
46
Osmium tetroxide fixative: for cytoplasmic structures
Flemmings without acetice acid
47
3 Fixatives for Electron Microscopy
Glutaraldehyde, Osmium tetroxide, Paraformaldehyde
48
2 Fixatives for Enzyme Histochemistry
4% Formaldehyde, formol saline
49
Fixative for Electron histochemistry and Electron immunocytochemistry
Karnovsky's paraformaldehyde-glutaraldehyde
49
Human brain must undergo intravascular perfusion and washed with?
Ringer's Lactate
49
Hard tissues (cervix, uterine, fibroid) must undergo what method?
Lendrum's method (immersing the spx in 4% aqueous phenol for 1-3 days)

Histopathology (Lecture) Pathology & Fixative (2 decks)

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