Fixatives ALCOHOL FIXATIVES

Question 1

● ADV: good for glycogen
● Causes polarization of glycogen (granules will move towards the poles or ends of the cells)
● DADV: dissolves FATs and LIPOPROTEINS

Answer 1

ALCOHOL FIXATIVES

Question 2

● Most rapid tissue fixative (1-3 hrs)
● Fixing brain tissue for rabies diagnosis
● Fixes Nissl granules and cytoplasmic granules

Answer 2

CARNOY’S FLUID

Question 3

● Enzyme studies; Does not fix but preserves glycogen

Answer 3

ETHANOL (70-100%)

Question 4

● Dry and wet smears, BM (bone marrow) smears, bacterial smears

Answer 4

METHANOL/WOOD ALCOHOL(100%)

Question 5

Touch prep smears to be Wright-stained

Answer 5

ISOPROPANOL

Question 6

For mucopolysaccharide (12-18 hrs)

Answer 6

NEWCOMER’S FLUID

Question 7

● For sputum (4-18 hrs)

Answer 7

GENDRE’S (ALCOHOLIC FORMALIN)