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Clinical Microscopy > Flow Cytometry > Flashcards

Flow Cytometry Flashcards

1
Q

Divine fluid or liquid window to the body’s inner workings

A

Urine

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2
Q

Urinalysis began ________

A

6000 years ago

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3
Q

What was urinalysis called 6000 years ago?

A

Uroscopy

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4
Q

Described bubbles on the surface of urine as an indication of kidney disease

A

Hippocrates

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5
Q

Bubbles in urine

A

Proteinuria

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6
Q

Collection of urine sample

A

Mid stream clean catch

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7
Q

How do you mix urine sample?

A

Gently invert

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8
Q

Volume of urine transferred into test tube

A

10 mL

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9
Q

Proper speed and time of centri for urine

A

400 relative centrifugal force for 5 minutes

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10
Q

Proper decanting of supernatant

A

Aspirate

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11
Q

Volume for slide prep of urine

A

0.2 mL

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12
Q

Minimum number of fields for microscopy

A

10/lpf or hpf

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13
Q

Kamukhang kamukha ng RBC

A

Yeast cells

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14
Q

Analysis of the physiologic and chemical properties of cells and other biological particles

A

Cytometry

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15
Q

Sysmex dye for urinalysis

A

Polymethine dye (fluorescent dye; stains nucleic acid)

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16
Q

Principle ng sysmex

A

Hydrofocusing

17
Q

Size: S-L

NO FLUORESCENCE

A

Crystals

18
Q

Size: S-M

Low fluorescence

A

RBC

19
Q

Very low size

Low low fluorescence

A

Bacteria

20
Q

Small size

Medium fluorescence

A

Sperm

21
Q

Small size

Low to medium fluorescence

A

Yeast like cells

22
Q

Largest in size

Highest fluorescence

A

WBC

23
Q

Small to big size

No fluorescence

A

Debris

24
Q

2 graphics used

A

Scattergram

Histogram

25
Q

Positive dipstick not only i dicates presence of RBC but also ___ and ____

A

Hgb and myoglobin

26
Q

Red or redbrown and clear urine is due to

A

Hgb and myoglobin

27
Q

3 aspects of urinalysis in the lab

A

Macroscopic
Chemical
Microscopic

28
Q

Fully automated urine analyzer

A

UX-2000

29
Q

Name of speaker

A

Zykhar Batulan

Clinical Microscopy (3 decks)

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