fluorescence Flashcards
(29 cards)
1
Q
applications
A
2
Q
quantum yield
A
3
Q
morse curve
A
4
Q
spec
A
light scatters=> better data at 90 degrees
only fraction of signal received
5
Q
solvent effect
A
6
Q
sample prep
A
7
Q
blanks and control
A
8
Q
measurement
A
9
Q
troubleshooting
A
10
Q
excitation spectra
A
11
Q
measuring exc spec
A
12
Q
interpreting spec
A
13
Q
inner filter effect
A
14
Q
self-Q
A
15
Q
dynamic vs static Q
A
16
Q
calcein leakage
A
17
Q
laurdan
A
solvent sensitive dye
as M melts more hydrophilic and fluid
18
Q
laurdan 2
A
19
Q
Ligand binding app
A
20
Q
Spec titration
A
21
Q
tight binding
A
22
Q
N determination
A
23
Q
alternative way to get N
A
24
Q
moderate binding
A
25
N for moderate or weak binding
26
2nd exp
27
non-linear fitting
28
titration with indicator
29
