food tests for biological molecules - big to small Flashcards

1
Q

what is the test for starch?

A

iodine solution

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2
Q

explain the test for starch

A
  1. place food sample on a spotting tile
  2. add a few drops of iodine solution to the sample
  3. blue- black colour indicates starch
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3
Q

what is the test for glucose / reducing sugars?

A

benedicts solution

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4
Q

explain the test for glucose / reducing sugars?

A
  1. place food sample in a boiling tube
  2. add benedict’s solution to the sample of food in solution
  3. place in a water bath at 80•c for 5 minutes
  4. a change from blue to brick red if present
    (if green, yellow or orange means less sugar present)
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5
Q

what is the test for protein?

A

biuret test

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6
Q

explain the test for protein

A
  1. place food sample in a boiling tube
  2. add enough biuret solution for colour to be pale blue
  3. if protein is present colour will change to mauve / purple
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7
Q

what is the test for lipids?

A

ethanol test

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8
Q

Explain the test for lipids?

A
  1. Place food sample in a test tube
  2. Add small amount of ethanol and shake to dissolve any lipid in the alcohol.
  3. Add equal volume of water
  4. Cloudy white colour ( emulsion will form) indication presence of lipid
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