Free linivg amoeba

Question 1

بسم الله الرحمن الرحيم
__________________
Mention free lving aboema causing Enceplaiits ?

Answer 1

-Neglaria folweri
-Acanthabmoeba

Question 2

بسم الله الرحمن الرحيم
__________________
Mention free lving aboema causing Enceplaiits ?

Answer 2

-Neglaria folweri
-Acanthabmoeba
facultative parasites

Question 3

Intro
free linving amoeba:-
Inhabit ,,,,,,,and,,,,,,,,,
No ,,,,,,, no ,,,,,,,no,,,,,,,,,
NO human to human transmission
No relation with saniatation

Answer 3

Soil and water

no definitive host no Resvoir no Vector

Question 4

Mention morphology of Neglaria Fowleri ?

Answer 4

2 Stages Trophozoites+cyst
3 froms :
Ameboid trophozite
Flagellate Trophozite
Cyst

Question 5

Invective stage of Neglarai fowelri ? >

Answer 5

Ameboid tropohzoites

Question 6

Invective stage of Neglarai fowelri ? >

Answer 6

Ameboid tropohzoites

Question 7

Diagnostic Stage of Neglaria fwoelir?

Answer 7

Ameboid trophozoites
dividing form
only form found in tissue and CSF

Question 8

Mention size and shape of Ameboid trophozite of NF

Answer 8

Size 7-20 micro
Shape :
broad end with peusdopodium called lopopodia
Narrow end

Question 9

Describe nucleous and Cytoplasm of Ambeoid cyst of Neglaria fowlair ?

Answer 9

Nucelus : large central vesicular with Central Karyosome + no peripheral chromatin
Cytoplasm : Clear ectoplasm
+ Granular endoplasm with contractile vacuoles

Question 10

What is PAM what is causing it?

Answer 10

Primary Ambeoic meningocencephalitis
By Neglaria Fowaleri !

Question 11

Flagellated trophozoites is
,,,,,,,and…..and………
formed when amobeis stage is exposed to change in ,,,,,?
It changes to ameboid form when there is unfavrable conditions in water before ,,,,?

Answer 11

Temporary , non dividing , nonfeeding
______________________
water ionization
___________________
Encysation

Question 12

Describe shape and nuscleus of Flagellate trophozoites

Answer 12

It pear like with 2 flagellats
Nulceus :
large vesicular with central karyosome and no peripheral chrmoatins

Question 13

Resistant stage of Neglaria Fowlei?

Answer 13

Cyst stage
7-15 micro in size

Question 14

Why cyst cannot be seen in sepicemins ?
and describe its shape + Nucelus

Answer 14

Because the course of the disease is Rapid and fatal even before trophozite encyst
shape : spherical with dobule wall with pore
Nucelus with single Nuceloulus

Question 15

Habitiat of N,fowleri ?

Answer 15

Soil and infected water
Human brain

Question 16

Mode of transmisson of PAM ?
Primary amboeic menigionencephalitis

Answer 16

1-Swimming and diving in infected water containing Amboeid trophozoites of N.fowleri
through nose to cribriform plate and olfactory nerve to brain cause acute suppuraive inflammation.
2-Inhalation of aerosols containing the Trophozites

Question 17

PAM caused but secnodary casued by?

Answer 17

PAM by N.fwoleir
Secondary entamboeica histolytica

Question 18

Pathogenisis of nagelria fowleri describe

Answer 18

Enter through nose to
cribirofom plate to
olfactory nerve
to brain and meiniges
casuing acute suppurative meningoencephlaitis
in perivascular spaces

Question 19

N,fowleeri has 2 ways to kill cells how ?

Answer 19

1-Trogocytosis : Ingestion of cytoplasm
2-Contact dependent lysis=acute necrotizing meninigoencephalitis

Question 20

C/P OF Naglarie fowelri ?
commonly affecting, ,,,,and,,,,
IP = …TO….
ONSET= ACUTE = ?

Answer 20

Chidlren and young adults
___________________
2-5 days
__________________
Tase and smell loss
Headache +Fever
Increased ICP
nECK rgigidit
Motor and sensory changes
Coma and death

Question 21

How to diagnose Naglaria fowleir?

Answer 21

History
CSF
Culture
CBC
PCR

Question 22

History for diagnosis of Naglareira fowleir?

Answer 22

Swimmming in infected water
Taste and smell changes differetiating it from ? Bacterial meningits

Question 23

Lumbar puncture in PAM reveal ?

Answer 23

Ameboid trophoziote
the CSF cc ?
Cloudy + Purulent + Blood tinged + Tension
with Increased Polymorpnucelar cells + lymphocytes + proetin
and Decreased glucose

Question 24

Culture of Nagleria FOwlei?

Answer 24

In non nutreint agar with gram negativ bacilli culture before

Question 25

CBC IN nagleria why?

Answer 25

To detect polymorphonueclarcells increase so differentiaed form viral encephalitis

Question 26

Treatment and prevention OF PAM DESCRIBE

Answer 26

ORALLY + Amphoteriin B IV _____________________ Avoid swimming in infected water Biguandies + bugacil to swimming pools

Question 27

GAE ! granulomatous ameobic encephalitis caused by?

Answer 27

Acanthameoba

Question 28

GAE ! granulomatous ameobic encephalitis caused by?

Answer 28

Acanthameoba or can cause amobic Keratitis it is Opportuistic !!!!!

Question 29

حتي إذا استيئس الرسل وظنوا أنهم قد كُذِبوا جاءهم نصرنا فنجي من نشاء ولا يُرَد بأسنا عن القوم المجرمين

Question 30

Mention Acanthamboea forms

Answer 30

Trophozite and cyst both are diagnostic and infective

Question 31

Trophozite of acantheabomba is ,,,,,,AND,,,,,,STAGE? SIZE :...TO..... SHAPE:.......... Nucleus:? cytoplasm ?

Answer 31

Infective and diagnosti 22-30 micro Irregualr with spine pseudopodia nucelus single with central karyosoma and no peripheral chromatin Cytopalsm contians single Contractile vacuole

Question 32

Cyst form of acantheamboeba is ,,,,and,,,,,,stage size? shape? Nucleus?? disperesed by ? to ? Acantheamboea cysts can be found in ,,,,,, in contrast to ?

Answer 32

Infective and diagnostic stage size =18 micro shape ? Rounded in shape with double wall the outer wall is wrinkeled and the inner wall is polyhedral Nucleus is single Dispetred by wind to contaminate environmetn Can be found in human tissue in contrast to Nagelaria

Question 33

habitat of acantheaombea?

Answer 33

Is water soil and contact lens or dialysis machine _____________ in human Brain + eye Lungs Genitourinary + Skin

Question 34

Mode of acanthamboeba?

Answer 34

Through corneal abraisons Trough nasal passages when inhalation aeroslos of cysts and trophozoites Through Skin abraisons Through Genitourinary tracts

Question 35

Pathogenisis of GAE granulomatius amboeci encephlaits

Answer 35

in immunocomprised or in healhty in organs away immunity as cornea _____________________ Via blood to brain with slow invasion of brain tissue causing chronic granulomatous inflammation and foci of granuloma with Hge and Necrois via 2 methods ? Torgocytosis eating cytoplasm Contact depedntn lysis

Question 36

Clincal presenation of Granulomatous abomeic enceplatiics ?

Answer 36

Gradual onset! weeks for monts is the incubation 1-symptoms and sign of increased icp ? Headche fever nausea vomiting Sensory and motor changes skin lesios as ulcerative nodules _______________________ Amboebic Keratitis : In using OF contaminaed contact lens causing abrasions of eye : Corneal ulcer Congested Conjunctive Corneal opacity and blindeness ___________________________________ Dissaminated: Skin ulcers and nodules in body may reach ear and eye , Genioturinaty tract and internal organs

Question 37

Diagnosis of GAE

Answer 37

Clinical : Contact eye lenses _____________________ Lab investiagtions :- 1-CSF 2-Culture 5-brain biopsy 3-Corneal scarppin 4-skin biopsy searching for what Trophozoite and Cysts of Acanthamobeba

Question 38

For treatments of GAE AND Amobic keratitis ?

Answer 38

GAE: Sulfadiazine + Ketoconazole _______________________ Amoebic Keratitis :- Chlor-hexadine Polyhexamethylene-Biguanide ________________________

Question 39

PREVENTION OF AMBOCI KEARTIS THEN GAE?

Answer 39

wearing clean Conatct lenses Avoiding waring lenses during swimming

Question 40

" الله نزل أحسن الحديث كتابا متشابها مثاني تقشعر منه جلود الذين يخشون ربهم "! .....

Answer 40

ثم تلين جلودهم وقلوبهم إلي ذكر الله ذلك هدي الله يهدي به من يشاء " :)