Fresh Tissue Exam Flashcards
view protoplasmic
activities
FRESH TISSUE
is the process of examining sections or sediments,
whereby cellular materials are spread lightly over a slide by means of a
wire loop or applicator,
Smear Preparation
useful in cytological examinations,
particularly for cancer diagnosis.
Smear Preparation
spx is immersed in a watch glass with an
isotonic saline solution (dissected or separated)
TEASING OR DISSOCIATION
~small pieces of tissue (not ≥ 1mm) placed in 2 mx
slides and compressed forcibly
Crushed prep
Recommended sps: for spreading
-Fresh sputum
-Bronchial aspirations
- Thick mucoid secretions
This method is a little more tedious than streaking, but has the advantage of maintaining cellular interrelationships of the material to be examined.
Spreading
This is useful for preparing smears of thick secretions such as serous fluids, concentrated sputum, enzymatic lavage samples from the gastrointestinal tract, and blood smears.
Pull apart
a special method of
smear preparation whereby the surface of a freshly cut piece of
tissue is brought into contact and pressed on to the surface of a clean glass slide, allowing the cells to be transferred directly to the
slide for examination by Phase Contrast microscopy o
Touch Preparation (Impression Smear)
It has an added advantage in that the cells may be examined without destroying their actual intercellular relationship, and without separating them from their normal surroundings.
Touch Preparation (Impression Smear) —
utilized when a rapid
diagnosis of the tissue in question is required,
FROZEN SECTIONS
Cryostat temperature
-10 to -20 deg.Celsius
size cut in microtome with C02 or on a Cryostat
10-15 u
generally used in histochemistry and during operative procedures, and is the most rapid of the commonly available freezing agents.
Liquid nitrogen
liquid at room temperature.
Isopentane
