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Gas Liquid chromatography Flashcards

(6 cards)

1
Q

What is the stationary phase?

A

Where the molecules can’t move, must be a solid or a liquid on a solid support
In this case, oil which coats a porous support inside a long tube

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2
Q

What is the mobile phase?

A

Where the molecules can move, always a liquid or a gas.
In this case it’s an inert (unreactive) carrier gas such as nitrogen or helium.

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3
Q

How does solubility affect retention time in GLC?

A

If a substance is more soluble in the mobile phase, then the retention time will be smaller, vice versa.

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4
Q

How does the temperature of the GLC machine effect retention time?

A

The hotter the GLC machine the more time the substance remains in the evaporated state, therefore moving through the tube quicker, reducing retention time.

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5
Q

How does boiling point affect retention time?

A

If a substance has a high boiling point, it’s more likely to travel through as a liquid rather than gas, therefore increasing retention time.

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6
Q

Why is combining GLC with mass spec a useful tool for analysis?

A

It makes it easy to identify the components of a mixture, as GLC is very good at separating liquids, and mass spec allows us to identify compounds.

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