Gastroenterology Labs Flashcards
(42 cards)
Why do we do a GI tract work up
Why do we do a GI tract work up
-direct visualization (endoscopy and colonoscopy) can be expensive and invasive
-dyspepsia
-ulcer disease- MC cause h. pylori
-celiac disease
-GI tract bleeding
-colorectal cancer
dyspepsia causes
Causes:
- GERD
- peptic ulcer ds
-ulcer disease
- gastritis
-H. pylori
H. pylori diagnostic lab tests
Biopsy during endoscopy - MOST ACCURATE DX
-Histology
-Urease enzyme - H. pylori produces this
-PCR
- grow culture
Breath test: Non-invasive
-Urea labeled with radioactive carbon
-If urease is present the urea will be split into ammonia and radioactive carbon
-Test can also be used to prove eradication
Stool test: H. pylori antigens
celiac disease
Descrption:
-immune mediated disorder
-triggered by gluten
Who to test:
- pts with malabsorption
- first degree relative
- elevated aminotransferase level (AST/ALT)
-type 1 DM with GI symptoms
Tests:
-IgA tTG antibodies test with total IgA > Sen and Spec of 98% -> if you have a very high suspicion you can still bx if neg
-Biopsy for confirmation (gold standard)
-if neg workup: consider non-celiac gluten sensitivity
approach to celiac disease disorder
ORDER IgA tTG with total IgA: dont need to know specifics
-basically if neg and still high sus -> bx
-if positive-> still bx
-IgA tTG + and total IgA normal -> small bowel bx
-IgA tTG and total IgA normal -> unlikely dx -> if suspect celiac disease remains do a bx
-IgA deficiency -> order IgG deaminated gliadin peptide -> if + -> small bowel bx
-> if neg and suspect celiac disease remains -> small bowel bx
upper GI bleeding causes
Causes:
-CANCER
-Esophageal varices- caput medusa, ascites
-Peptic ulcer disease- NSAIDs use
-GERD
-Gastritis
-Duodenitis
lower GI bleeding causes
Causes:
-CANCER (colorectal)
-Hemorrhoids
-Anal fissures
-Inflammatory bowel disease- know diff between crohns and ulcerative colitis
-Diverticulitis
Ulcerative Colitis vs Crohns
UC:
- colon and rectum
- only the innermost lining of the colon -> bloody
- continuous inflammation
- bloody diarrhea
Crohns:
- mouth to anus (mostly small bowel)
- skip lesions/patchy inflammation
- transmural
GI bleed work up
1) endoscopy or colonoscopy for biopsy
2) CBC- check for anemia
3) fecal occult blood test
colorectal cancer (2nd leading cause of death) tests
Colonoscopy- every 10 years
Fecal immunochemical test (FIT) with DNA:
- sensitivity 79% (20-30% missed with a false negative!)
- test annually
Fecal occult blood test
- only 20-50% identified with colon cancer
CT colonography
flexible sigmoidoscopy
liver function
Conjunction- bilirubin
Metabolism- thyroid and steroid hormones
Storage- amino acids, carbohydrates, lipids, vitamins, minerals
Synthesis:
- protein (proteins LFTs, albumin)
- coagulation factors (2,5,7,9,10)
- transport proteins
- bile acids from cholesterol
- PT/INR elevated with liver disease due to decreased coagulation factors
Excretory
Detoxification : drugs and toxins
“ChatGPT Makes Studying Seriously Extremely Difficult”
- Conjunction
- Metabolism
- Storage
- Synthesis
- Excretory
- Detoxification
direct vs indirect bilirubin
Indirect (unconjugated = not water soluble)
- bilirubin in the blood ; NOT URINE
- elevated in hemolytic anemia, genetic disorders,
Direct (conjugated)
- hepatocytes done the work and packaged; problem is after liver synthesis
- can be excreted: dark urine
- elevated in liver disease, obstructions in liver, gall bladder, pancreatitis
-pancreatic cancer- extrahepatic blockage
liver: excretory function
-bilirubin comes from broken down hemolysis of RBCs (90-120 days)
-recycle it in liver
-unconjugated -> conjugated bilirubin -> excreted into the bile
-store in galbladder
-bile: aid in digestion of lipids
AST: aspartate animotransferase when is it decreased and increased
- shows signs of hepatocellular damage: AST/ALT elevated when liver is “screaming”
Decreased:
- liver congestion
- high cholesterol
Increased:
- liver ds
- alcohol abuse
- MI: AST
- kidney infection/disease
ALT: alanine aminotransferase
- indicates hepatocellular damage
- more specific for liver function
- elevated: liver ds
AST : ALT ratio
ETOH induced liver disease: AST> ALT
- AST:ALT value greater than 2
- SLAST: alcohol liver issue
… when specific to liver ALT is higher but with alcohol AST is higher
liver enzymes: lactate dehydrogenase (LDH) when is it elevated
elevated:
- cardiac
- RBC hemolysis
- renal disease
-could be many things
-non specific
Hepatic shock
Hepatic shock: acute liver dysfunction from lack of blood flow
- ill patients
-more common in pts with hemodynamic disorders
-preventable with early treatment of underlying disease.
-There is no definite treatment -> manage conservatively
-Hepatic shock in patients can increase the mortality rate
biliary tract: alkaline phosphatase (ALP)- when is it increased
Liver diseases:
-Cirrhosis
-Hepatitis
-Biliary obstruction
Bone Diseases:
-Bone Tumors
-Osteoporosis
-Rickets
-Padget’s disease- lytic lesions on all of bones
-Hyperparathyroidism
-Malignancies (leukemias & Lymphomas)
ALP:
-found in highest concentration in liver, bile ducts, and bone
ALK Phos - when is it decreased
DECREASED:
-Wilson’s Disease
-Hyperphosphatasia
-Aplastic Anemia
-Pernicious Anemia
-Cretinism
-Chronic myelogenous leukemia (CML)
WHAP - CC
ALP: indicates cholestasis, inflitrative ds, billiary obstruction
mc cancers that metastases to bone
-breast
-prostate
biliary tract: gamma-glutamltransferase (GGT) when is it increased vs decreased
“LLL PP B Elevate GGT -> consecutive GGt think the 3L, 2 P, B pattern”
-lung cancer
-liver ds
-lupus (Systemic lupus erythematosus)
-pancreatitis
-prostate cancer
-breast cancer
Decreased:
-hyperthyroidism
-hypothalamic dysfunction
Indicators of hepatocellular damage - which LFTs?
AST
ALT
LDH - non specific
Cholestasis or billary obstruction - which LFTs are elevated
ALP
GGT
Bilirubin
Bile acids
5’- nucleotidase
