Gel Electrophoresis

Question 1

What is gel electrophoresis primarily used for?

Answer 1

Gel electrophoresis is primarily used for separating nucleic acids or proteins based on their size.

Question 2

True or False: The size of the DNA fragments can affect their migration distance in gel electrophoresis.

Answer 2

True

Question 3

Fill in the blank: The gel matrix in gel electrophoresis is usually made from _______.

Answer 3

agarose or polyacrylamide

Question 4

What factor can influence the resolution of bands in gel electrophoresis?

Answer 4

The concentration of the agarose or polyacrylamide gel.

Question 5

Multiple Choice: Which of the following can cause smearing of bands in gel electrophoresis? A) Overloading the sample B) Using too low a voltage C) Proper sample preparation D) All of the above

Answer 5

A) Overloading the sample

Question 6

What does a ladder or marker in gel electrophoresis help to determine?

Answer 6

It helps to determine the size of the DNA fragments in the samples.

Question 7

True or False: Ethidium bromide is commonly used as a staining agent in gel electrophoresis.

Answer 7

True

Question 8

What is the purpose of running a control sample alongside experimental samples in gel electrophoresis?

Answer 8

To ensure the accuracy and reliability of the results.

Question 9

Fill in the blank: The migration of DNA fragments in gel electrophoresis is influenced by both size and _______.

Answer 9

the voltage applied