Gel Electrophoresis

Question 1

What is gel electrophoresis ?

Answer 1

Technique used to separate charged molecules like DNA /RNA and proteins according to their size

Question 2

Gel electrophoresis and DNA

Answer 2

Electrophoresis and DNA

Enables you to distinguish DNA fragments of different lengths

Question 3

History of gel electrophoresis

Answer 3

Discovered in the early 19th century not implemented till 1942

Question 4

Advantages of gel electrophoresis ?

Answer 4

gel easily poured does not denature the samples samples can also be recovered .

Question 5

Disadvantges

Answer 5

gels melt during electophoresus
Buffer can become exhausted
Different forms of genectic material may run in unpredictable forms

Question 6

Procedure

Answer 6

1. Preparing the samples
2. An agarose Tae gel solution is prepared
3. Casting the gel
4. Setting up the electrophoresis
5. Loading the gel
6. Electrophoresis
7. Stopping electrophoresis and visualizing the DNA

Question 7

Examples of gel electrophoresis

Answer 7

Tae agarose gel electrophoresis
TBE and denaturing PAGE are commons for RNA seperation
SDS page is a denaturing gel electrophoresis used for proteins

Question 8

Comparison

Answer 8

Tae agaraose gel another type of gel electrophoresis

Known as Tris - acetate - EDTAbuffer is used as both a running /in agarose gel

Used in denaturing gradient of gel electrophresis

Tae used to study the mobility of DNA in solution with and without sodium chloride

Similarity same in terms of actors , such as the gel could melt during the experience .