Gene Exchange by Conjugation Flashcards Preview

microbio ch 8.3 : genetic exchange & recombination > Gene Exchange by Conjugation > Flashcards

Flashcards in Gene Exchange by Conjugation Deck (4)
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1
Q

Cell-to-cell transfer of a plasmid

A

The original cell retains the
plasmid and one strand moves into the recipient cell (where
the other strand is replicated)

Also called mating but its not sexual reproduction!

2
Q

Requirements for conjugation:

Cell-to-cell contact between donor & recipient cells via a

A

pilus made by the donor cell

3
Q

Requirements for conjugation:

tra genes -

A

these encode the pilus and other apparatus
necessary for conjugation

4
Q

oriT (origin of transfer)

A

this is the site where rolling-circle
replication begins to make a copy of the plasmid DNA that
is exchanged with the neighboring cell (in contact via pilus)
8