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Molecular Biology > gene expression > Flashcards

gene expression Flashcards

1
Q

direction of template strand

A

3’ to 5’

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2
Q

direction of RNA polymerase

A

5’ to 3’

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3
Q

post trc modification of mRNA in prokarytes

A

doesnt happen

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4
Q

post trc modification of mRNA in eukaryotes

A

RNA splicing, 5’ end capping, addition of polyA tail

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5
Q

what is splicing?

A

the removal of introns and joining of exons

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6
Q

what are snRNPs

A

small nuclear RNA and proteins

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7
Q

what is spliceosome?

A

complex of snRNPS that remove introns from pre DNA

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8
Q

what is RNA capping?

A

covalent attachment of a modified guanosine to the 5’ end of the mRNA

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9
Q

when does RNA capping occur?

A

during transcription after the first 20-25 nts have been transcribed

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10
Q

what does RNA capping do?

A

allows proper exiting of mRNA from the nucleus to cytoplasm AND enables ribosome binding to mRNA

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11
Q

what does polyA tail do?

A

stabilizes mRNA in cytosol

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Molecular Biology (8 decks)

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