Gene Mainpulation Flashcards
(5 cards)
1
Q
How does Sanger work
A
Radio labelled bases that terminate chain
Gel electrophoresis
Read of bases
2
Q
How does Nanopore sequencing work
A
ssDNA forced thru nanopore
Current that passes thru gives what bases it is
3
Q
Which mAChR is needed for DA release
A
mAChR 4
4
Q
How to control Cre nuclear access
A
Fuse to OER- give tamoxifen it moves to nucleus
5
Q
How to control gene promoter
A
Put gene under tetracycline responsive promoter
