Gene Organisation and Transcription I Flashcards Preview

Nucleic Acid and Gene Expression > Gene Organisation and Transcription I > Flashcards

Flashcards in Gene Organisation and Transcription I Deck (17):
1

What are the genes that are expressed in all cells called?

Housekeeping genes

2

How far is each base pair separated by?

0.34 nm

3

Is all RNA translated? Examples?

No. tRNA, rRNA, and snRNA.

4

Which strand is transcribed, sense or antisense?

Antisense a.k.a. template

5

What regulates transcription and how?

Transcription factors by bending DNA (modifying histones and remodelling chromatin) and making certain genes more or less accessible

6

What does RNA polymerase I transcribe?

rRNA genes

7

What does RNA polymerase III transcribe?

tRNA and 5S RNA genes

8

What does RNA polymerase II transcribe?

mRNA

9

What is the DNA sequence at which the transcription complex assembles called?

Gene promoter

10

What does the Basal Transcription Complex allow and how?

A minimal level of transcription via phosphorylating RNA polymerase II

11

Describe the formation of the Basal Transcription Complex,

TFIID binds to TATA in the minor groove. TFIIA and TFIIB bind. RNA polymerase then binds to TFIIB with TFIIF bound onto it. TFIIE, TFIIH, TFIIJ all then bind to RNA polymerase II.

12

How many TF II 'X' bind during the formation of the Basal transcription complex and in what order?

7. D, A + B, polymerase + F, E + H + J

13

Why is TFIIB particularly important?

RNA polymerase binds to it

14

Which TF does RNA polymerase bind to?

TFIIB

15

What does TFIID consist of?

TATA binding protein (TBP) and TBP Accessory Factors (TAF's)

16

What does TFIIH do once bound?

Promotes further unwinding of the DNA and synthesis of RNA by phosphorylating RNA polymerase II

17

How do genes that need to be transcribed in one part of the body know where to be transcribed?

Transcriptions factors that regulate the expression of genes that need to be expressed in a certain part of the body are only made in that certain part of the body so that gene can only be expressed in that part of the body