Genetic control of metabolism Flashcards
1
Q
How can wild strains of DNA be improved
A
Mutagenesis (exposure to UV light) or recombinant DNA
2
Q
What is a vector
A
A DNA molecule used to carry foreign genetic info into another cell,
3
Q
What are used as vectors during recombinant DNA technology
A
Artificial chromosomes and plasmids
4
Q
What does restriction endonucleases doc
A
Cut open plasmids and specific genes out of chromosomes leaving sticky ends.
5
Q
What does ligase do
A
Seals the gene into the plasmid
6
Q
What do selectable markers do
A
Protect the micro organism from a selective agent that would normally kill it or prevent it from growing.
7
Q
What alternative cells should be used to prevent polypeptides being incorrectly folded
A
Recombinant Yeast cells
