GENETIC ENGINEERING Flashcards
(18 cards)
Carries the genetic information in all living organisms.
DNA
a single stranded type of nucleic acids that’s mostly involved in protein synthesis
RNA
is a problem molecule that catalyzes biochemical reaction.
enzymes
circular, double-stranded DNA molecule.
plasmid
facilitates the formation of the phosphodiester bond that forms the DNA structure. (Responsible for formation/bonding)
DNA ligase
is a process that uses laboratory-based technologies to alter the DNA makeup of an organism.
genetic engineering
genetic engineering is also called
genetic modification
2 types of genetic engineering
- classical breeding
- recombinant DNA technology
The practice of mating or breeding selected organisms with desirable.
classical breeding
4 steps in classical breeding
- Determine which trait are significant enough to be chosen.
- Select parents that exemplify the desirable traits.
- Choose the best offspring from parents to produce the next generation.
- Repeat the process.
examples of classical breeding
bulldog and shih tzu mix
guapple
wagyu beef
Is a series of techniques used to manipulate and isolate DNA segments of interest.
recombinant dna technology
5 steps of recombinant dna technology
- We need two variables DNA Containing the “gene of interest” and a Bacterial plasmid;
- We need to cut and open the Bacterial plasmid by “restriction enzyme”;
- Insert the gene of interest into the open plasmid by DNA ligase.
- Then you can now have RECOMBINANT DNA. Afterwards, Insert the Recombinant DNA to the bacterial cell.
- Then isolate the bacteria for it to multiply
recombinant dna technology examples
bacilus thuringiensis
human insulin
resistant to corn-borer disease
bacilus thuringiensis
resistant to eggplant fruit and shoot borer
bacilus thuringiensis
aims to fix a faulty gene or replace it with a healthy gene to try to cure disease or make the body better able to fight disease.
gene therapy
is a genetically modified (GM) rice that produces beta carotene, which the body converts into vitamin A.
golden rice