Genetic Technology Flashcards
(11 cards)
1
Q
agarose gel
A
used to separate DNA molecules of different sequence lengths
2
Q
polyacrylamide
A
use to separate proteins of various amino acid lengths
3
Q
blotting
A
used to detect molecules & preserve the same for later
4
Q
southern blotting
A
detection of specific DNA sequences
5
Q
Northern blotting
A
detects RNA
6
Q
western blotting
A
detects specific proteins
7
Q
PCR - polymerase chain reaction
what are the 3 steps used?
A
- denaturing - the DNA (98 degrees C)
- annealing - complimentary nucleotides called primes attach to the single stranded templates (50-65 degrees C)
- Extension - by attaching bases (72 degrees C)
8
Q
DNA cloning
A
used for gene amplification
9
Q
DNA sequencing
A
used for analysis of DNA
10
Q
Restriction fragment length polymorphism (RFLPs) `
A
restriction enzymes bind to & cut the dsDNA; a different fragment length lets you know there is a difference in the specific sequence within the individual
11
Q
Gel electrophoresis
A
separates molecules of different sizes using a charge gradient
