GENETICS Flashcards
(55 cards)
Which end does a nucleotide join
3’
What was the purpose of the Hershey Chase experiment
To determine the identity of the hereditary molecule
What was infected in the experiment
E.coli
What was DNA labelled with in the experiment
Phosphorus
What was protein labelled with
Sulfur
What was used to separate the mixture of bacteria and virus
Blender and centrifuge
What was the evidence that DNA is hereditary
After the solutions were seperated the tube that was marked with sulfur was radioactive at the top and the tube marked with phosphorus was radioactive at the bottom
What type of reaction occurs when a nucleotide is added
Dehydration reaction
What direction is the template strand read
3’ - 5’
Which direction is the daughter strand built
5’-3’
How much of the parent DNA is conserved
Half
What are origins of replication
Points along DNA where replication starts
What is a replication bubble
When the DNA unzips at origins of replication and becomes single stranded
Half of a replication bubble
replication fork
What is the order of enzymes in replication
Helicase topoisomerase SSB RNA primase DNA polymerase III DNA polymerase I DNA ligase DNA polymerase II
What does helices do
unzip DNA
What does topoisomerase do
Relieve tension in helix
What does SSB do
Stabilize the single strands
What does RNA primate do
Adds the primer
What does DNA polymerase III do
Binds to strand and starts replication by adding nucleotides
What does DNA polymerase I do
Remove primers and replace with DNA
What does DNA ligase do
Join Okazaki fragments to form phosphodiester bonds
What does DNA polymerase II do
check for errors
What strand replicates continuously
leading strand
