Genetics And Biotechnology

Question 0

Inactive densely packed chromatin during INTERPHASE

Answer 0

Heterochromatin

Question 1

Total amount of purine = amount of pyrimidine

Answer 1

Chargaffs rule

Question 2

Active, lightly stained chromatin

Answer 2

Euchromatin

Question 3

Histones are high in 2 types of amino acids

Answer 3

Arginine and lysine

Question 4

Phase of cell cycle: DNA replication

Answer 4

S phase

Question 5

Protein that recognize origin of replication

Answer 5

DNA A protein

Question 6

Unwinds the double helix

Answer 6

Helicase

Question 7

Maintains separation of parental strands

Answer 7

Single stranded DNA binding protein

Question 8

Remove supercoils

Answer 8

Tropoisomerase

Question 9

Cleaves both strand of supercoils

Target of quinolones

Answer 9

Gyrase

Question 10

Synthesize RNA (Primers)

Answer 10

Primase

Question 11

Catalyze chain elongation and proofreads

Answer 11

Polymerase 3

Question 12

Removes RNA primers

Answer 12

Polymerase 1

Question 13

Fills in gaps

Answer 13

Polymerase 1

Question 14

Seals in nicks between okazaki fragments

Answer 14

Ligase

Question 15

Enzyme found in HIV/ retroviruses

Answer 15

Reverse transcriptase

Question 16

Production of thymine dimers due to exposure to UV light

Answer 16

Xeroderma pigmentosa

Question 17

Start codon

Answer 17

AUG

Question 18

Antibiotic that binds to the 30s subunit

Answer 18

Stremtomycin

Question 19

Antibiotic that binds to the 50s subunit

Answer 19

Clindamycin and erythromycin

Question 20

Antibiotic that inhibits peptidyltransferase

Answer 20

Chloramphenicol

Question 21

Antibiotic that Binds tob subunit of bacterial DNA dependent RNA polymerase

Answer 21

Rifampicib

Question 22

Toxin that inactivates eEf-2

Answer 22

Diphtheria toxin

Question 23

Any heritable change in the DNA base sequence

Answer 23

Mutation

Question 24

Point mutation of purine-pyrimidine to purine-pyrimidine

Answer 24

Transition

Question 25

Point mutation of purine-pyrimidine to pyrimidine-purine

Answer 25

Transversion

Question 26

Change in 3rd position of codon that code for same amino acid

Answer 26

Silent mutation

Question 27

New codon is a stop codon

Answer 27

Nonsense

Question 28

New codon is a different amino acid

Answer 28

Missense

Question 29

Deletion or addition of a base

Answer 29

Frameshift mutation

Question 30

3 diseases associated with splice donor or acceptor

Answer 30

Tay sachs Gaucher B-thalassemia

Question 31

Triple repeat expansion: CAG

Answer 31

Huntington

Question 32

Triple repeat expansion: CCG

Answer 32

Fragile X syndrome

Question 33

Triple repeat expansion: CDG

Answer 33

Myotonic dystrophy

Question 34

Used to deduce original sequence of DNA

Answer 34

Sanger DNA sequencing

Question 35

Used to synthesize many copies of desired fragment of DNA

Answer 35

PCR

Question 36

Used to determine which restriction fragment of DNA are associated with a particular gene. A DNA sample is is placed on a gel

Answer 36

Southern blot

Question 37

Involves radioactive DNA probe binding to sample RNA

Answer 37

Northern blot

Question 38

Measure amount of antigen or antibody

Answer 38

Western blot

Question 39

Nucleic acid sequences are arranged in grids on glass or silicon

Answer 39

Microarrays

Question 40

Biothechnology used in localization of genes and direct visualization of anomalies at molecular level

Answer 40

FISH

Question 41

Diagnostic tool for diseases involving single- base changes or deletions/ insertions of DNA into a restriction fragment

Answer 41

Restriction fragment length polymorphism

Question 42

Production of recombinant DNA molecule that is self perpetuating

Answer 42

Cloning

Question 43

Treatment option for diseases caused by deficiency of a gene product

Answer 43

Gene therapy