genome nd variants fa (clive) Flashcards

(22 cards)

1
Q

histone proteins function in chromatin compaction

A

h1

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2
Q

The 2 amino acids residue
found in the periphery of your
histone proteins.

A

lysine & arginine

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2
Q

Gene or Intergenic Region
Introns
Promoter
SINES
DMTRs

A

Introns - Gene
Promoter Region - Gene
SINES - IR
DMTRs - IR

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3
Q

RNA or DNA

Paramyxoviridae
Filoviridae
Poxviridae
Papillomaviridae
Parvoviridae

A

Paramyxoviridae - RNA
Filoviridae - RNA
Poxviridae - DNA
Papillomaviridae - DNA
Parvoviridae - DNA

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4
Q

Prokaryotes or Eukaryotes

Circular
Linear
Naked
Stored in the nucleoid
Stored in chromosomes

A

Circular - Prokaryote
Linear - Eukaryote
Naked - Prokaryote
Nucleoid - Prokaryote
Chromosomes - Eukaryote

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5
Q

size of your solenoid?

A

30 nm

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5
Q

Among the histone core
proteins, which has the most
number of isomers?

A

h2a

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6
Q

Classify if the ribosome
indicated is Eukaryotes or
Prokaryotes:
60S
40S
50S
30S
28S
18S
23S

A

60S - Eukaryotes
40S - Eukaryotes
50S - Prokaryotes
30S - Prokaryotes
28S - Eukaryotes
18S - Eukaryotes
23S - Prokaryotes

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7
Q

Among all of the histone
proteins, which has the most
number of isomers?

A

h1

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8
Q

In a nucleosome, how many base pairs of DNA are
present?

A

147-150 bp

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9
Q

How many base pairs of DNA
are bound in your linker
proteins?

A

80 bp

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10
Q

Among the nucleoid
associated proteins in
bacteria, which of the
following recruits RNA
polymerase into the bacterial
chromosome?

A

Integration Host Factors
(IHF)

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11
Q

From what organisms were
the Polytene Chromosomes
isolated?

A

Drosophila melanogaster
(fruit fly)

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12
Q

From what organism is the
Lampbrush Chromosome
isolated?

A

Urodele Amphibians
● Salamanders

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13
Q

Between the Polytene and
Lampbrush Chromosomes,
which of the following is
described to be a giant
chromosome that is
characterized in replicating
DNA minus the cell
replication.

A

Polytene Chromosome

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14
Q

Between the Polytene and
Lampbrush Chromosomes,
which is better used for gene
expression studies?

A

Lampbrush Chromosome

14
Q

T/F
Your aceto-orcein is a
fluorescent stain in visualizing
Giant Chromosomes.

A

False
Aceto-orcein is used for
brightfield microscopy
Propidium iodide is used
for fluorescent tag

15
Q

The acronym SINES stands
for what?

A

Short Interspersed
Nuclear Elements

16
Q

These are non-repetitive DNA
that codes for functional RNA
but do not translate to
Proteins.

A

Non-coding genes

17
Q

The acronym LINES stands
for what?

A

Long Interspersed
Nuclear Elements

18
Q

What region in the central
dogma do pseudogenes lack
compared to actual functional
genes?

A

Promoter region

19
Q

Between SINES and LINES,
what comprises 20-21% of
the genome?