GIGUERE - LECTURE 6 Flashcards
(40 cards)
what does RNA pol II transcribe
messenger RNA and long non coding RNAs
general structure of RNA pol II
what does RNA pol II cooperate with and what do those do
cooperates with GTFs (general transcription factors)
recognise and open promoter DNA
initiate RNA synthesis
stimulate escape of RNA pol II from the promoter
what does TFIIA do
what does TFIIB do
what does TFIID do
what does TFIIE do
what does TFIIF do
what does TFIIH contain
what does initiation complex consist of
RNA polymerase II and the GTFs at promoter DNA
very complex process
what do RNA pol II and GTFs need to do at transcription initiation
what is the size of the transcriptional initiation complex
> 1 megadalton in size and occupies >100 base pairs around the TSS
what are the steps of PIC assembly
what happens in the 1st step (recognition of the promoter)
what happens in the second step (set the TSS and bend DNA)
what happens in the third step (RNA pol II is recruited to form the core PIC)
what happens in the 4th step (the DNA is opened by helicase activity, the PIC is closed)
what happens in the 5th step
what happens in the 6th step
what happens in the 7th step
what does the closed vs open complex look like
what are core promoters
important: not all elements are present at each promoter
many genes do not have any of these core promoter elements
molecular mechanisms remain to be fully defined
what is the TATA box
what is the INR (initiator)
INR=iniator element
